A chiral ligand exchange ce essay with zinc(ii)-l-valine complex for determining enzyme kinetic constant of l-amino acid oxidase
文献类型：期刊论文
| 作者 | Qi, Li1,2,4; Yang, Gengliang4; Zhang, Haizhi1,2,3; Qiao, Juan1,2,3 |
| 刊名 | Talanta
 |
| 出版日期 | 2010-06-15 |
| 卷号 | 81期号:4-5页码:1554-1559 |
| 关键词 | Enzyme kinetics L-amino acid oxidase Ligand exchange ce Zinc complex Enantioseparation |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2010.03.001 |
| 通讯作者 | Qi, li() |
| 英文摘要 | A new strategy for the enantioseparation of d,l-amino acids employing the principle of ligand exchange capillary electrophoresis with zn(ii)-l-valine complex as a chiral selecting system in the presence of beta-cyclodextrin has been designed. successful enantioseparation of label flee and labeled amino acids have been achieved with a buffer of 100 0 mm boric acid, 5 0 mm ammonium acetate, 4.0 mm beta-cyclodextrin, 4 0 mm znso(4) and 8 0 mm l-valine at ph 8.1 this new method was shown to be applicable to the quantitative analysis of label free d- and l-aromatic amino acids furthermore, the expanding enzymatic use of l-amino acid oxidase to incubate with different l-amino acids has allowed understanding of the substrate's specificity. an on-column incubation assay has been developed to study the l-amino acid oxidase's catalytic efficiency. it was demonstrated that the enzyme kinetic constant could be determined by using this new method (c) 2010 elsevier b v. all rights reserved. |
| WOS关键词 | CAPILLARY-ELECTROPHORESIS METHOD ; ZONE-ELECTROPHORESIS ; BETA-CYCLODEXTRIN ; SEPARATION ; RESOLUTION ; VENOM ; ENANTIOSEPARATION ; DERIVATIZATION ; ENANTIOMERS ; DERIVATIVES |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000278737100062 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2405431 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 4.Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Li,Yang, Gengliang,Zhang, Haizhi,et al. A chiral ligand exchange ce essay with zinc(ii)-l-valine complex for determining enzyme kinetic constant of l-amino acid oxidase[J]. Talanta,2010,81(4-5):1554-1559. |
| APA | Qi, Li,Yang, Gengliang,Zhang, Haizhi,&Qiao, Juan.(2010).A chiral ligand exchange ce essay with zinc(ii)-l-valine complex for determining enzyme kinetic constant of l-amino acid oxidase.Talanta,81(4-5),1554-1559. |
| MLA | Qi, Li,et al."A chiral ligand exchange ce essay with zinc(ii)-l-valine complex for determining enzyme kinetic constant of l-amino acid oxidase".Talanta 81.4-5(2010):1554-1559. |
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来源：中国科学院大学
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