Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi
文献类型：期刊论文
| 作者 | Liu, Yanqun1,2; Li, Yuping1; Wang, Huan1; Xia, Runxi1; Li, Xisheng2; Wan, Haolei3,4; Qin, Li1; Jiang, Defu2; Lu, Cheng5; Xiang, Zhonghuai5 |
| 刊名 | Acta biochimica et biophysica sinica
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 42期号:11页码:816-826 |
| 关键词 | Antheraea pernyi Enolase Cloning Expression pattern |
| ISSN号 | 1672-9145 |
| DOI | 10.1093/abbs/gmq084 |
| 通讯作者 | Liu, yanqun(liuyqlyp@yahoo.com.cn) |
| 英文摘要 | In this study, two enolase genes were isolated and characterized from the chinese oak silkworm, antheraea perny, which were designated as enolase i and ii, respectively. the enolase i cdna sequence was 1712 bp with an open reading frame (orf) of 1302 bp encoding 433 amino acids. the enolase ii cdna sequence was 1549 bp with an orf of 1296 bp encoding 431 amino acids. the amino acid sequences of the two genes share several conserved features/sites of enolase. antheraea pernyi enolase i shows 93%-97% sequence identity to enolases of lepidopterans available to date, 75%-82% identity to enolases of other invertebrates, 60%-72% identity to enolases of other organisms including vertebrates, plants, and fungi. antheraea pernyi enolase ii shows 84% identity to bombyx mori enolase ii, but 60% identity to a. pernyi enolase i. in the phylogenetic tree, enolase ii sequences from a. pernyi and b. mori were clearly separated from the majority of enolase sequences of higher organisms including a. pernyi and b. mori enolase i sequences. by sequence comparisons and phylogenetic analysis, we suggest that enolase ii from a. pernyi and b. mori may be a new member of the enolase superfamily. antheraea pernyi enolase i mrna was found in all tested tissues whereas enolase ii mrna was expressed specifically in the spermaries and ovaries, suggesting that the product of enolase ii gene may be related to reproduction. the transcript abundance of a. pernyi enolase i gene was significantly down-regulated after cold shock and significantly up-regulated after heat shock, suggesting that a. pernyi enolase i gene may be inducible by temperature stress. |
| WOS关键词 | HUMAN ALPHA-ENOLASE ; NUCLEOTIDE-SEQUENCE ; MOLECULAR-CLONING ; GENOME SEQUENCE ; ANGSTROM RESOLUTION ; CRYSTAL-STRUCTURE ; BOMBYX-MORI ; PROTEIN ; EVOLUTION ; STRESS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000283657600008 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2405620 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Yanqun |
| 作者单位 | 1.Shenyang Agr Univ, Coll Biosci & Biotechnol, Dept Sericulture, Shenyang 110866, Peoples R China 2.Sericultural Inst Liaoning Prov, Fengcheng 118100, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100029, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 5.Southwest Univ, Minist Agr, Key Sericultural Lab, Chongqing 400716, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yanqun,Li, Yuping,Wang, Huan,et al. Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi[J]. Acta biochimica et biophysica sinica,2010,42(11):816-826. |
| APA | Liu, Yanqun.,Li, Yuping.,Wang, Huan.,Xia, Runxi.,Li, Xisheng.,...&Xiang, Zhonghuai.(2010).Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi.Acta biochimica et biophysica sinica,42(11),816-826. |
| MLA | Liu, Yanqun,et al."Cdna cloning and expression pattern of two enolase genes from the chinese oak silkworm, antheraea pernyi".Acta biochimica et biophysica sinica 42.11(2010):816-826. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。