Br regulates the expression of the ecdysone biosynthesis gene npc1
文献类型：期刊论文
| 作者 | Xiang, Yanhui1,2; Liu, Zhonghua1; Huang, Xun1 |
| 刊名 | Developmental biology
 |
| 出版日期 | 2010-08-15 |
| 卷号 | 344期号:2页码:800-808 |
| 关键词 | Npc1 Br Ecdysone Ring gland Drosophila |
| ISSN号 | 0012-1606 |
| DOI | 10.1016/j.ydbio.2010.05.510 |
| 通讯作者 | Huang, xun(xhuang@genetics.ac.cn) |
| 英文摘要 | The growth and metamorphosis of insects are regulated by ecdysteroid hormones produced in the ring gland. ecdysone biosynthesis-related genes are both highly and specifically expressed in the ring gland. however, the intrinsic regulation of ecdysone biosynthesis has received little attention. here we used the drosophila npc1 gene to study the mechanism of ring gland-specific gene expression. npc1 is important for sterol trafficking in the ring gland during ecdysone biosynthesis. we have identified a conserved ring gland-specific cis-regulatory element (rse) in the npc1 promoter using promoter fusion reporter analysis. furthermore, genetic loss-of-function analysis and in vitro electrophoretic mobility shift assays revealed that the ecdysone early response gene broad complex (br) is a vital factor in the positive regulation of npc1 ring gland expression. moreover, br also affects the ring gland expression of many other ecdysone biosynthetic genes as well as torso and inr, two key factors in the regulation of ecdysone biosynthesis. these results imply that ecdysone could potentially act through its early response gene br to achieve positive feedback regulation of ecdysone biosynthesis during development. (c) 2010 elsevier inc. all rights reserved. |
| WOS关键词 | INDUCED PUFFING CASCADE ; BROAD-COMPLEX ; DROSOPHILA-MELANOGASTER ; STEROL HOMEOSTASIS ; PROTHORACIC GLAND ; MOLTING HORMONE ; SALIVARY-GLANDS ; CASPASE DRONC ; P450 ENZYME ; METAMORPHOSIS |
| WOS研究方向 | Developmental Biology |
| WOS类目 | Developmental Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000280787100024 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2405694 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Xun |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Yanhui,Liu, Zhonghua,Huang, Xun. Br regulates the expression of the ecdysone biosynthesis gene npc1[J]. Developmental biology,2010,344(2):800-808. |
| APA | Xiang, Yanhui,Liu, Zhonghua,&Huang, Xun.(2010).Br regulates the expression of the ecdysone biosynthesis gene npc1.Developmental biology,344(2),800-808. |
| MLA | Xiang, Yanhui,et al."Br regulates the expression of the ecdysone biosynthesis gene npc1".Developmental biology 344.2(2010):800-808. |
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来源：中国科学院大学
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