Distinction of single base mismatches in duplex dna using methylene blue as optical indicator
文献类型：期刊论文
| 作者 | Zhang, Zhanxia1,2; Wang, Xiaolei1; Wang, Yong1,2; Yang, Xiurong1 |
| 刊名 | Analyst
 |
| 出版日期 | 2010 |
| 卷号 | 135期号:11页码:2960-2964 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c0an00359j |
| 通讯作者 | Yang, xiurong(xryang@ciac.jl.cn) |
| 英文摘要 | A simple and sensitive approach for the recognition of single base mismatches in duplex dna was developed. the single base mismatched double-strand (ds) dna and the completely complementary dsdna can quench the fluorescence of methylene blue to the different values, so the point mutation dsdna can be identified from the duplex dna effectively. the fluorescence intensity was decreased with the increasing dsdna concentration in the range of 10 similar to 1000 nm, the detection limit was estimated to be 50 nm (s/n = 3). the contrast experiment showed that this method possesses the universality for the distinction of other single base mismatches in duplex dna. moreover, the interaction mechanism between methylene blue and dsdna was also discussed in detail. the uv-visible absorption and the fluorescence experiments indicated that the interaction of methylene blue and dsdna had at least two effective modes: the electrostatic binding and the intercalation binding. when more methylene blue molecules bound onto mismatched dsdna, the fluorescence intensity of methylene blue would decrease to a lower value than that of completely complementary dsdna. therefore, single base mismatched dsdna can be recognized from duplex dna effectively. |
| WOS关键词 | FLUORESCENCE SPECTROSCOPY ; NUCLEOTIDE POLYMORPHISMS ; AMPLIFIED DETECTION ; HYBRIDIZATION ; SEQUENCE ; PROBE ; IDENTIFICATION ; TRANSDUCTION ; BINDING |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000283138100030 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2405743 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, Xiurong |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Elect Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhanxia,Wang, Xiaolei,Wang, Yong,et al. Distinction of single base mismatches in duplex dna using methylene blue as optical indicator[J]. Analyst,2010,135(11):2960-2964. |
| APA | Zhang, Zhanxia,Wang, Xiaolei,Wang, Yong,&Yang, Xiurong.(2010).Distinction of single base mismatches in duplex dna using methylene blue as optical indicator.Analyst,135(11),2960-2964. |
| MLA | Zhang, Zhanxia,et al."Distinction of single base mismatches in duplex dna using methylene blue as optical indicator".Analyst 135.11(2010):2960-2964. |
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来源：中国科学院大学
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