Study on amino amides and enzyme kinetics of l-asparaginase by mce
文献类型：期刊论文
| 作者 | Qiao, Juan1,2; Qi, Li1; Ma, Huimin1; Chen, Yi1; Wang, Meixiang1; Wang, Dexian1 |
| 刊名 | Electrophoresis
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 31期号:9页码:1565-1571 |
| 关键词 | Amino amides Enzyme kinetic constant L-asparaginase Mce |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200900520 |
| 通讯作者 | Qi, li(qili@iccas.ac.cn) |
| 英文摘要 | Seven kinds of amino amides including three synthetic arylglycine amides and four normal amino amides were successfully separated by mce with lip detector. using micellar electrokinetic electrophoresis, the optimized separation of the seven kinds of amino amides was achieved with fitc as the labeling reagent and polyoxyethylene lauryl ether as the surfactant in 20.0 mm borate at ph 9.2. under the optimized conditions, linearity of l-asparagine was obtained in the range of 6.6 x 10(-6)-2.6 x 10(-4)m; the detection limit (s/n = 3) was 0.7 mu m. the enzyme kinetic constants of l-asparaginase using l-asparagine as substrate were also determined by this method and the kinetic parameter k(m) and v(max) for the enzymatic hydrolysis of l-asparagine were 442.0 mu m and 69.9 mu m/min, respectively. |
| WOS关键词 | CAPILLARY-ELECTROPHORESIS ; MICROCHIP ELECTROPHORESIS ; SEPARATION ; ACIDS ; CHROMATOGRAPHY ; LEUKEMIA ; ASSAY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000277844500020 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2405917 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Juan,Qi, Li,Ma, Huimin,et al. Study on amino amides and enzyme kinetics of l-asparaginase by mce[J]. Electrophoresis,2010,31(9):1565-1571. |
| APA | Qiao, Juan,Qi, Li,Ma, Huimin,Chen, Yi,Wang, Meixiang,&Wang, Dexian.(2010).Study on amino amides and enzyme kinetics of l-asparaginase by mce.Electrophoresis,31(9),1565-1571. |
| MLA | Qiao, Juan,et al."Study on amino amides and enzyme kinetics of l-asparaginase by mce".Electrophoresis 31.9(2010):1565-1571. |
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来源：中国科学院大学
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