Proteome reference map and comparative proteomic analysis between a wild type clostridium acetobutylicum dsm 1731 and its mutant with enhanced butanol tolerance and butanol yield
文献类型：期刊论文
| 作者 | Mao, Shaoming2; Luo, Yuanming1; Zhang, Tianrui; Li, Jinshan; Bao, Guanhui2; Zhu, Yan2; Chen, Zugen3; Zhang, Yanping; Li, Yin1; Ma, Yanhe1 |
| 刊名 | Journal of proteome research
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 9期号:6页码:3046-3061 |
| 关键词 | Clostridium acetobutylicum Proteome reference map Comparative proteome analysis Two-dimensional gel electrophoresis Acidogenesis Solventogenesis Butanol tolerance Butanol yield |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/pr9012078 |
| 通讯作者 | Li, yin(yli@im.ac.cn) |
| 英文摘要 | The solventogenic bacterium clostridium acetobutylicum is an important species of the clostridium community. to develop a fundamental tool that is useful for biological studies of c. acetobutylicum, we established a high resolution proteome reference map for this species. we identified 1206 spots representing 564 different proteins by mass spectrometry, covering approximately 50% of major metabolic pathways. to better understand the relationship between butanol tolerance and butanol yield, we performed a comparative proteomic analysis between the wild type strain dsm 1731 and the mutant rh8, which has higher butanol tolerance and higher butanol yield. comparative proteomic analysis of two strains at acidogenic and solventogenic phases revealed 102 differentially expressed proteins that are mainly involved in protein folding, solvent formation, amino acid metabolism, protein synthesis, nucleotide metabolism, transport, and others. hierarchical clustering analysis revealed that over 70% of the 102 differentially expressed proteins in mutant rh8 were either upregulated (e.g., chaperones and solvent formation related) or downregulated (e.g., amino acid metabolism and protein synthesis related) in both acidogenic and solventogenic phase, which, respectively, are only upregulated or downregulated in solventogenic phase in the wild type strain. this suggests that rh8 cells have evolved a mechanism to prepare themselves for butanol challenge before butanol is produced, leading to an increased butanol yield. this is the first report on the comparative proteome analysis of a mutant strain and a base strain of c. acetobutylicum. the fundamental proteomic data and analyses will be useful for further elucidating the biological mechanism of butanol tolerance and/or enhanced butanol production. |
| WOS关键词 | CORYNEBACTERIUM-GLUTAMICUM ; TRANSCRIPTIONAL ANALYSIS ; LACTOCOCCUS-LACTIS ; ESCHERICHIA-COLI ; MOLECULAR CHARACTERIZATION ; RECOMBINANT STRAINS ; SOLVENT PRODUCTION ; LIPID-COMPOSITION ; ATCC 824 ; PROTEINS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| WOS记录号 | WOS:000278243300025 |
| 出版者 | AMER CHEMICAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2406035 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Yin |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China 3.Univ Calif Los Angeles, Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA |
| 推荐引用方式 GB/T 7714 | Mao, Shaoming,Luo, Yuanming,Zhang, Tianrui,et al. Proteome reference map and comparative proteomic analysis between a wild type clostridium acetobutylicum dsm 1731 and its mutant with enhanced butanol tolerance and butanol yield[J]. Journal of proteome research,2010,9(6):3046-3061. |
| APA | Mao, Shaoming.,Luo, Yuanming.,Zhang, Tianrui.,Li, Jinshan.,Bao, Guanhui.,...&Ma, Yanhe.(2010).Proteome reference map and comparative proteomic analysis between a wild type clostridium acetobutylicum dsm 1731 and its mutant with enhanced butanol tolerance and butanol yield.Journal of proteome research,9(6),3046-3061. |
| MLA | Mao, Shaoming,et al."Proteome reference map and comparative proteomic analysis between a wild type clostridium acetobutylicum dsm 1731 and its mutant with enhanced butanol tolerance and butanol yield".Journal of proteome research 9.6(2010):3046-3061. |
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来源：中国科学院大学
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