The sumo pathway functions in mouse oocyte maturation
文献类型：期刊论文
| 作者 | Wang, Zhen-Bo1,2; Ou, Xiang-Hong1,4; Tong, Jing-Shan1; Li, Sen1,2; Wei, Liang1,2; Ouyang, Ying-Chun1; Hou, Yi1; Schatten, Heide3; Sun, Qing-Yuan1 |
| 刊名 | Cell cycle
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 9期号:13页码:2640-2646 |
| 关键词 | Sumoylation Mouse oocyte maturation Overexpression Senp2 Mii spindle |
| ISSN号 | 1538-4101 |
| DOI | 10.4161/cc.9.13.12120 |
| 通讯作者 | Sun, qing-yuan(sunqy@ioz.ac.cn) |
| 英文摘要 | Sumoylation is an important posttranslational modification in which sumo (small ubiquitin-related modifier) proteins are bonded covalently to their substrates. studies on the roles of sumoylation in cell cycle regulation have been emerging in both mitosis from yeast to mammals and meiosis in budding yeast, but the functions of sumoylation in mammalian meiosis, especially in oocyte meiotic maturation are not well known. here, we examined the localization and expression of sumo-1 and sumo-2/3, the two basic proteins in the sumoylation pathway and investigated their roles through overexpression of senp2 during mouse oocyte maturation. immunofluorescent staining revealed differential patterns of sumo-1 and sumo-2/3 localization: sumo-1 was localized to the spindle poles in prometaphase i, mi and mii stages, around the separating homologues in anaphase i and telophase i stages of first meiosis, while sumo-2/3 was mainly concentrated near centromeres during mouse oocyte maturation. immunoblot analysis uncovered the different expression profiles of sumo-1 and sumo-2/3 modified proteins during mouse oocyte maturation. overexpression of senp2, a sumo-specific isopeptidase, caused changes of sumo-modified proteins and led to defects in mii spindle organization in mature eggs. these results suggest that the sumo pathway may play an indispensable role during mouse oocyte meiotic maturation. |
| WOS关键词 | CELL-CYCLE PROGRESSION ; KINASE CDC5 ACT ; TOPOISOMERASE-II ; ISOPEPTIDASE SMT4/ULP2 ; CHROMOSOME SEGREGATION ; REGULATE SUMOYLATION ; SYNAPTONEMAL COMPLEX ; CONJUGATING ENZYME ; OPPOSING FASHION ; NUCLEAR-PORE |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000281205400038 |
| 出版者 | LANDES BIOSCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2406047 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, Qing-Yuan |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA 4.So Med Univ, Nanfang Hosp, Dept Obstet & Gynecol, Ctr Reprod Med, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhen-Bo,Ou, Xiang-Hong,Tong, Jing-Shan,et al. The sumo pathway functions in mouse oocyte maturation[J]. Cell cycle,2010,9(13):2640-2646. |
| APA | Wang, Zhen-Bo.,Ou, Xiang-Hong.,Tong, Jing-Shan.,Li, Sen.,Wei, Liang.,...&Sun, Qing-Yuan.(2010).The sumo pathway functions in mouse oocyte maturation.Cell cycle,9(13),2640-2646. |
| MLA | Wang, Zhen-Bo,et al."The sumo pathway functions in mouse oocyte maturation".Cell cycle 9.13(2010):2640-2646. |
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来源：中国科学院大学
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