Detection of hbv-dna in dried bloodstains on filter paper by nested polymerase chain reaction
文献类型：期刊论文
| 作者 | Zhang, Jie1; Zhang, Ling1; Song, Manshu1; Wang, Wei1,2,3 |
| 刊名 | Labmedicine
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 41期号:9页码:535-539 |
| 关键词 | Nested pcr Chelex 100 Bloodstains Hbv-dna |
| ISSN号 | 0007-5027 |
| DOI | 10.1309/lmgvuubetvjnx91y |
| 通讯作者 | Wang, wei(wei6014@yahoo.com) |
| 英文摘要 | Objective: to describe the technical performance of nested pcr for identifying hepatitis b viral (hbv) dna. methods: hepatitis b viral dna was extracted from a dried bloodstain on filter paper by a chelex-100 method. then the dna fragment was amplified by nested polymerase chain reaction (pcr). the sensitivity and specificity of this method were also analyzed. the positive rate of the nested pcr-based method was compared with that of the enzyme-linked imnnunosorbent assays (elisa) method. results: the lowest detection limit of the test was 5 copies of hbv dna per pl by this method. mcnemar's test showed that the difference between the positive rates of these 2 methods was not statistically significant (p=0.289, p>0.05). hepatitis b viral test results showed a good concordance between these 2 methods (kappa=0.727). conclusion: a very small amount of the dried blood sample is required for the detection, which could overcome the shortage of the normal elisa method that requires a relatively large amount of fresh blood samples. |
| WOS关键词 | HEPATITIS-B-VIRUS ; BLOOD SPOTS ; REACTION AMPLIFICATION ; EXTRACTION METHOD ; PCR ; CHELEX-100 |
| WOS研究方向 | Medical Laboratory Technology |
| WOS类目 | Medical Laboratory Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000281262500004 |
| 出版者 | AMER SOC CLINICAL PATHOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2406489 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Wei |
| 作者单位 | 1.Capital Med Univ, Sch Publ Hlth & Family Med, Beijing, Peoples R China 2.Chinese Acad Sci, Coll Life Sci, Grad Univ, Beijing, Peoples R China 3.Curtin Univ Technol, Sch Publ Hlth, Perth, WA, Australia |
| 推荐引用方式 GB/T 7714 | Zhang, Jie,Zhang, Ling,Song, Manshu,et al. Detection of hbv-dna in dried bloodstains on filter paper by nested polymerase chain reaction[J]. Labmedicine,2010,41(9):535-539. |
| APA | Zhang, Jie,Zhang, Ling,Song, Manshu,&Wang, Wei.(2010).Detection of hbv-dna in dried bloodstains on filter paper by nested polymerase chain reaction.Labmedicine,41(9),535-539. |
| MLA | Zhang, Jie,et al."Detection of hbv-dna in dried bloodstains on filter paper by nested polymerase chain reaction".Labmedicine 41.9(2010):535-539. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。