Circulating microrna: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans
文献类型：期刊论文
| 作者 | Wang, Guo-Kun1,2,3,4; Zhu, Jia-Qi1; Zhang, Jun-Tao2,3,4; Li, Qing2,3,4,6; Li, Yue1; He, Jia5; Qin, Yong-Wen1; Jing, Qing1,2,3,4 |
| 刊名 | European heart journal
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 31期号:6页码:659-666 |
| 关键词 | Microrna Mir-208a Blood Acute myocardial infarction Biomarker |
| ISSN号 | 0195-668X |
| DOI | 10.1093/eurheartj/ehq013 |
| 通讯作者 | Qin, yong-wen(ywqin1@yahoo.com.cn) |
| 英文摘要 | Microrna (mirna) is reported to be present in the blood of humans and has been increasingly suggested as a biomarker for diseases. we aim to determine the potential of cardiac-specific mirnas in circulation to serve as biomarkers for acute myocardial infarction (ami). by verifying their tissue expression patterns with real-time polymerase chain reaction (pcr) analysis, muscle-enriched mirnas (mir-1, mir-133a, and mir-499) and cardiac-specific mir-208a were selected as candidates for this study. with mirna microarray and real-time pcr analyses, mir-1, mir-133a, and mir-499 were present with very low abundance, and mir-208a was absent in the plasma from healthy people. in the ami rats, the plasma levels of these mirnas were significantly increased. especially, mir-208a in plasma was undetected at 0 h, but was significantly increased to a detectable level as early as 1 h after coronary artery occlusion. further evaluation of the mirna levels in plasma from ami patients (n = 33) demonstrated that all four mirna levels were substantially higher than those from healthy people (n = 30, p < 0.01), patients with non-ami coronary heart disease (n = 16, p < 0.01), or patients with other cardiovascular diseases (n = 17, p < 0.01). notably, mir-208a remained undetectable in non-ami patients, but was easily detected in 90.9% ami patients and in 100% ami patients within 4 h of the onset of symptoms. by receiver operating characteristic curve analysis, among the four mirnas investigated, mir-208a revealed the higher sensitivity and specificity for diagnosing ami. elevated cardiac-specific mir-208a in plasma may be a novel biomarker for early detection of myocardial injury in humans. |
| WOS关键词 | ANIMAL DEVELOPMENT ; CANCER ; MICROVESICLES ; EXPRESSION ; DISEASE ; PLASMA ; MARKER ; BLOOD ; RATS ; RNA |
| WOS研究方向 | Cardiovascular System & Cardiology |
| WOS类目 | Cardiac & Cardiovascular Systems |
| 语种 | 英语 |
| WOS记录号 | WOS:000275817900010 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2406837 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Qin, Yong-Wen |
| 作者单位 | 1.Changhai Hosp, Dept Cardiol, Shanghai 200433, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Lab Nucle Acid & Mol Med, Shanghai, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200030, Peoples R China 5.Second Mil Med Univ, Dept Hlth Stat, Shanghai, Peoples R China 6.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Guo-Kun,Zhu, Jia-Qi,Zhang, Jun-Tao,et al. Circulating microrna: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans[J]. European heart journal,2010,31(6):659-666. |
| APA | Wang, Guo-Kun.,Zhu, Jia-Qi.,Zhang, Jun-Tao.,Li, Qing.,Li, Yue.,...&Jing, Qing.(2010).Circulating microrna: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans.European heart journal,31(6),659-666. |
| MLA | Wang, Guo-Kun,et al."Circulating microrna: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans".European heart journal 31.6(2010):659-666. |
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来源：中国科学院大学
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