Analysis of the vaccine potential of a laboratory escherichia coli strain in a japanese flounder model
文献类型：期刊论文
| 作者 | Cheng, Shuang1,2; Jiao, Xu-dong1,2; Zhang, Min1,2; Sun, Li1 |
| 刊名 | Fish & shellfish immunology
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 28期号:2页码:275-280 |
| 关键词 | Aeromonas hydrophila Cross-protection Dh5 alpha Escherichia coli Vibrio harveyi |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2009.11.003 |
| 通讯作者 | Sun, li(lsun@ms.qdio.ac.cn) |
| 英文摘要 | Escherichia coli dh5 alpha is a genetically tailored laboratory strain that is commonly used for general cloning. in this study, the vaccine potential of dh5 alpha was investigated. it was found that when used as a live vaccine, dh5 alpha could afford effective protection upon japanese flounder against aeromonas hydrophila infection. vaccination with purified outer membrane proteins and lipopolysaccharides of dh5 alpha failed to induce protective immunity against a. hydrophila. specific antibody production was observed in fish immunized with dh5 alpha, which lasted at least 8 weeks and was enhanced by a booster injection during the vaccination process. analysis of the transcription profiles of immune-related genes showed that vaccination with dh5 alpha heightened the expression of the genes encoding factors that are likely involved in both specific and nonspecific immunities. furthermore, compared to the control fish, fish vaccinated with dh5 alpha/paq1, which is dh5 alpha harboring the plasmid paq1 that expresses the coding element of a vibrio harveyi antigen, exhibited significantly improved survival rates following v harveyi and a. hydrophila challenges. these results demonstrate that dh5 alpha possesses intrinsic immunoprotective potential against a. hydrophila. this property, together with the feature of cloning friendliness, should render dh5 alpha useful in the construction of cross-protective vaccines. (c) 2009 elsevier ltd. all rights reserved. |
| WOS关键词 | CU/ZN SUPEROXIDE-DISMUTASE ; OUTER-MEMBRANE PROTEINS ; LIVE ATTENUATED VACCINE ; INDIAN MAJOR CARP ; AEROMONAS-HYDROPHILA ; PROTECTIVE IMMUNITY ; EDWARDSIELLA-TARDA ; FISH ; PATHOGENICITY ; SALMONICIDA |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000274721300004 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2407105 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Shuang,Jiao, Xu-dong,Zhang, Min,et al. Analysis of the vaccine potential of a laboratory escherichia coli strain in a japanese flounder model[J]. Fish & shellfish immunology,2010,28(2):275-280. |
| APA | Cheng, Shuang,Jiao, Xu-dong,Zhang, Min,&Sun, Li.(2010).Analysis of the vaccine potential of a laboratory escherichia coli strain in a japanese flounder model.Fish & shellfish immunology,28(2),275-280. |
| MLA | Cheng, Shuang,et al."Analysis of the vaccine potential of a laboratory escherichia coli strain in a japanese flounder model".Fish & shellfish immunology 28.2(2010):275-280. |
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来源：中国科学院大学
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