Cloning and expression profiles of two isoforms of a chh-like gene specifically expressed in male chinese shrimp, fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Li, Shihao1,2; Li, Fuhua1; Wang, Bing1; Xie, Yusu1,2; Wen, Rong1,2; Xiang, Jianhai1 |
| 刊名 | General and comparative endocrinology
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 167期号:2页码:308-316 |
| 关键词 | Crustacean hyperglycemic hormone (chh) Cloning Expression In situ hybridization Spermatophore sac Fenneropenaeus chinensis |
| ISSN号 | 0016-6480 |
| DOI | 10.1016/j.ygcen.2010.03.028 |
| 通讯作者 | Li, fuhua(fhli@ms.qdio.ac.cn) |
| 英文摘要 | Two full-length cdna sequences (fc-chh1, fc-chh2) encoding a crustacean hyperglycemic hormone (chh) precursor homolog and their dna sequences were cloned from chinese shrimp fenneropenaeus chinensis. the deduced amino acid sequences of them are predicted to contain a signal peptide and a mature peptide. the mature peptides of fc-chh1 and fc-chh2 shared 78% identity, but they showed low identities (less than 40%) to chh peptides from other species. both fc-chh1 and fc-chh2 proteins contain six highly conserved cysteine residues which are characteristic of the chh family peptides. the transcripts of fc-chh1 and fc-chh2 were shown to be specifically present in the spermatophore sac of mature male chinese shrimp through reverse transcription-polymerase chain reaction (rt-pcr) detection. the transcripts of fc-chh1 and fc-chh2 begin to appear at the immature stage (115 days after the first post-larvae stage) when the spermatophore sac was first observed to be appeared. in situ hybridization analyses showed that fc-chh1 and fc-chh2 transcripts located at the epithelial cells in the internal wall of the spermatophore sac. in the cloned dna sequences of fc-chh1 and fc-chh2, the predicted transcription factor binding sites in the 5' flanking sequences are different from those previously reported for chh family genes of crustacean. to our knowledge, these are novel chh-like genes expressed specifically in male shrimp. their function needs to be further investigated. (c) 2010 elsevier inc. all rights reserved. |
| WOS关键词 | CRUSTACEAN HYPERGLYCEMIC HORMONE ; MOLT-INHIBITING HORMONE ; CRAB CHIONOECETES-OPILIO ; MOLECULAR-CLONING ; CARCINUS-MAENAS ; REPRODUCTIVE PRODUCTS ; DECAPOD CRUSTACEANS ; X-ORGAN ; FAMILY ; CELLS |
| WOS研究方向 | Endocrinology & Metabolism |
| WOS类目 | Endocrinology & Metabolism |
| 语种 | 英语 |
| WOS记录号 | WOS:000278172500014 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2407135 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shihao,Li, Fuhua,Wang, Bing,et al. Cloning and expression profiles of two isoforms of a chh-like gene specifically expressed in male chinese shrimp, fenneropenaeus chinensis[J]. General and comparative endocrinology,2010,167(2):308-316. |
| APA | Li, Shihao,Li, Fuhua,Wang, Bing,Xie, Yusu,Wen, Rong,&Xiang, Jianhai.(2010).Cloning and expression profiles of two isoforms of a chh-like gene specifically expressed in male chinese shrimp, fenneropenaeus chinensis.General and comparative endocrinology,167(2),308-316. |
| MLA | Li, Shihao,et al."Cloning and expression profiles of two isoforms of a chh-like gene specifically expressed in male chinese shrimp, fenneropenaeus chinensis".General and comparative endocrinology 167.2(2010):308-316. |
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来源：中国科学院大学
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