Screening and characterization of butanol-tolerant micro-organisms
文献类型：期刊论文
| 作者 | Li, J.2,3; Zhao, J. B.1,2; Zhao, M.2,3; Yang, Y. L.1,2; Jiang, W. H.1,2,4; Yang, S.1,2,4 |
| 刊名 | Letters in applied microbiology
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 50期号:4页码:373-379 |
| ISSN号 | 0266-8254 |
| 关键词 | Butanol tolerance Chemostat Lab Relative growth rate Screening |
| DOI | 10.1111/j.1472-765x.2010.02808.x |
| 通讯作者 | Yang, s.(syang@sibs.ac.cn) |
| 英文摘要 | Aims: poor butanol tolerance of solventogenic stains directly limits their butanol production during industrial-scale fermentation process. this study was performed to search for micro-organisms possessing elevated tolerance to butanol. methods and results: two strains, which displayed higher butanol tolerance compared to commonly used solventogenic clostridium acetobutylicum, were isolated by evolution and screening strategies. both strains were identified as lactic acid bacteria (lab). on this basis, a lab culture collection was tested for butanol tolerance, and 60% of the strains could grow at a butanol concentration of 2 center dot 5% (v/v). in addition, an isolated strain with superior butanol tolerance was transformed using a certain plasmid. conclusions: the results indicate that many strains of lab possessed inherent tolerance of butanol. significance and impact of the study: this study suggests that lab strains may be capable of producing butanol to elevated levels following suitable genetic manipulation. |
| WOS关键词 | CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824 ; LACTOBACILLUS-PLANTARUM ; ETHANOL TOLERANCE ; LIPID-COMPOSITION ; ESCHERICHIA-COLI ; SELECTION ; ACETONE ; METABOLISM ; MEMBRANE ; BACTERIA |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| 出版者 | WILEY-BLACKWELL PUBLISHING, INC |
| WOS记录号 | WOS:000275396300008 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2407224 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, S. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Res Ctr Ind Biotechnol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Synthet Biol, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China 4.Shanghai Res & Dev Ctr Ind Biotechnol, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, J.,Zhao, J. B.,Zhao, M.,et al. Screening and characterization of butanol-tolerant micro-organisms[J]. Letters in applied microbiology,2010,50(4):373-379. |
| APA | Li, J.,Zhao, J. B.,Zhao, M.,Yang, Y. L.,Jiang, W. H.,&Yang, S..(2010).Screening and characterization of butanol-tolerant micro-organisms.Letters in applied microbiology,50(4),373-379. |
| MLA | Li, J.,et al."Screening and characterization of butanol-tolerant micro-organisms".Letters in applied microbiology 50.4(2010):373-379. |
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来源：中国科学院大学
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