The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic t-lymphocyte epitopes
文献类型：期刊论文
| 作者 | Liu, Jun1,2,3; Sun, Yeping1,2,3; Qi, Jianxun1,3; Chu, Fuliang1; Wu, Hao4; Gao, Feng5; Li, Taisheng6,7,8; Yan, Jinghua1,3; Gao, George F.1,2,3,9 |
| 刊名 | Journal of infectious diseases
 |
| 出版日期 | 2010-10-15 |
| 卷号 | 202期号:8页码:1171-1180 |
| ISSN号 | 0022-1899 |
| DOI | 10.1086/656315 |
| 通讯作者 | Gao, george f.(gaof@im.ac.cn) |
| 英文摘要 | Background. severe acute respiratory syndrome coronavirus (sars-cov), which emerged with highly contagious and life-threatening characteristics in 2002, remains a potential risk for future outbreaks. membrane (m) and envelope (e) proteins are major structural proteins of the sars-cov. the m protein has been determined as a protective antigen in humoral responses. however, its potential roles in stimulating cellular immunity remain elusive. methods. in this study, a panel of peptides derived from m and e proteins were tested by in vitro refolding, t2 cell-binding assays, and responses stimulated by cytotoxic t-lymphocyte (ctl) epitopes in hla-a2.1/k(b) transgenic mice and human peripheral blood mononuclear cells (pbmcs). results. a nonameric epitope mn2 and a decameric epitope md3 derived from the m protein were identified and used for the evaluation of m protein-specific immunity. responses stimulated by m protein-specific ctl epitopes have been found in the pbmcs of donors who had recovered from sars infection. additionally, the transmembrane domain of the m protein may contain a t cell epitope cluster revealed by the immunogenic and structural analysis of a panel of truncated peptides overlapping with mn2 and md3. conclusions. the m protein of sars-cov holds dominant cellular immunogenicity. this, together with previous reports of a strong humoral response against the m protein, may help to further explain the immunogenicity of sars and serves as potential targets for sars-cov vaccine design. |
| WOS关键词 | RECOVERED SARS PATIENTS ; CELL RESPONSES ; HONG-KONG ; CRYSTAL-STRUCTURES ; TRANSGENIC MICE ; SPIKE PROTEIN ; CTL EPITOPES ; CLASS-I ; IDENTIFICATION ; PEPTIDE |
| WOS研究方向 | Immunology ; Infectious Diseases ; Microbiology |
| WOS类目 | Immunology ; Infectious Diseases ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000288250900005 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2407379 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, George F. |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Mol Microbiol, Beijing 100101, Peoples R China 4.Capital Med Univ, You An Hosp, Dept Infect Dis, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 6.Beijing Union Med Coll Hosp, Dept Infect Dis, Beijing, Peoples R China 7.Chinese Acad Med Sci, AIDS Res Ctr, Beijing 100037, Peoples R China 8.Peking Union Med Coll, Beijing 100021, Peoples R China 9.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jun,Sun, Yeping,Qi, Jianxun,et al. The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic t-lymphocyte epitopes[J]. Journal of infectious diseases,2010,202(8):1171-1180. |
| APA | Liu, Jun.,Sun, Yeping.,Qi, Jianxun.,Chu, Fuliang.,Wu, Hao.,...&Gao, George F..(2010).The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic t-lymphocyte epitopes.Journal of infectious diseases,202(8),1171-1180. |
| MLA | Liu, Jun,et al."The membrane protein of severe acute respiratory syndrome coronavirus acts as a dominant immunogen revealed by a clustering region of novel functionally and structurally defined cytotoxic t-lymphocyte epitopes".Journal of infectious diseases 202.8(2010):1171-1180. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。