Clpc1, an atp-dependent clp protease in plastids, is involved in iron homeostasis in arabidopsis leaves
文献类型：期刊论文
| 作者 | Wu, Huilan1; Ji, Yanyan1; Du, Juan1,2; Kong, Danyu1,2; Liang, Hui1; Ling, Hong-Qing1 |
| 刊名 | Annals of botany
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 105期号:5页码:823-833 |
| 关键词 | Arabidopsis Clpc1 Iron homeostasis Chloroplasts Clp protease |
| ISSN号 | 0305-7364 |
| DOI | 10.1093/aob/mcq051 |
| 通讯作者 | Ling, hong-qing(hqling@genetics.ac.cn) |
| 英文摘要 | Background and aims iron (fe) is necessary for plant growth and development. although it is well known that fe deficiency causes chlorosis in plants, it remains unclear how the fe homeostasis is regulated in mesophyii cells. the aim of this work was to identify a gene related to fe homeostasis in leaves. methods a spontaneous mutant irm1, which revealed typical fe-deficiency chlorosis, was found from arabidopsis thaliana. using map-based cloning, the gene responsible for the altered phenotype of irm1 was cloned. the expression of genes was analysed using northern blot hybridization and multiplex rt-pcr analysis. further, gus staining with transgenic promoter-gus lines and transient expression of the fusion protein with gfp were used for detecting the expression pattern of the gene in different tissues and at different developmental stages, and for the subcelluar localization of the gene product. key results a point mutation from g to a at nucleotide 2317 of clpc1 on chromosome v of arabidopsis is responsible for the irm1 phenotype. the leaf chlorosis of the mutant irm1 and clpc1 (a t-dna-inserted null mutant of clpc1) could be converted to green by watering the soil with fe solution. the expression intensity of ferric reductase fro8 in irm1 and clpc1 was disordered (significantly higher than that of wild type). conclusions the glycine residue at amino acid 773 of clpc1 is essential for its functions. in addition to its known functions reported previously, clpc1 is involved in leaf fe homeostasis, presumably via chloroplast translocation of some nuclear-encoded proteins which function in fe transport. |
| WOS关键词 | FERRIC-CHELATE REDUCTASE ; MOLECULAR CHAPERONE ; METAL TRANSPORTER ; GENE FAMILY ; IN-VIVO ; PLANTS ; THALIANA ; DEFICIENCY ; EXPRESSION ; GROWTH |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000277729800018 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2407694 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Ling, Hong-Qing |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Huilan,Ji, Yanyan,Du, Juan,et al. Clpc1, an atp-dependent clp protease in plastids, is involved in iron homeostasis in arabidopsis leaves[J]. Annals of botany,2010,105(5):823-833. |
| APA | Wu, Huilan,Ji, Yanyan,Du, Juan,Kong, Danyu,Liang, Hui,&Ling, Hong-Qing.(2010).Clpc1, an atp-dependent clp protease in plastids, is involved in iron homeostasis in arabidopsis leaves.Annals of botany,105(5),823-833. |
| MLA | Wu, Huilan,et al."Clpc1, an atp-dependent clp protease in plastids, is involved in iron homeostasis in arabidopsis leaves".Annals of botany 105.5(2010):823-833. |
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来源：中国科学院大学
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