Identification and inactivation of pleiotropic regulator ccpa to eliminate glucose repression of xylose utilization in clostridium acetobutylicum
文献类型：期刊论文
| 作者 | Ren, Cong1,2; Gu, Yang1; Hu, Shiyuan1,2; Wu, Yan1,2; Wang, Pan1; Yang, Yunliu1; Yang, Chen1; Yang, Sheng1,3; Jiang, Weihong1,3 |
| 刊名 | Metabolic engineering
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 12期号:5页码:446-454 |
| 关键词 | Clostridium acetobutylicum Disruption of ccpa D-glucose/d-xylose mixture utilization |
| ISSN号 | 1096-7176 |
| DOI | 10.1016/j.ymben.2010.05.002 |
| 通讯作者 | Yang, sheng(syang@sibs.ac.cn) |
| 英文摘要 | D-xylose utilization is a key issue for lignocellulosic biomass fermentation, and a major problem in this process is carbon catabolite repression (ccr). in this investigation, solvent-producing bacterium clostridium acetobutylicum atcc 824 was metabolically engineered to eliminate d-glucose repression of d-xylose utilization. the ccpa gene, encoding the pleiotropic regulator ccpa, was experimentally characterized and then disrupted. under ph-controlled conditions, the ccpa-disrupted mutant (824ccpa) can use a mixture of d-xylose and d-glucose simultaneously without ccr. moreover, this engineered strain produced acetone, butanol and ethanol (abe) at a maximal titer of 4.94, 12.05 and 1.04 g/l, respectively, which was close to the solvent level of maize- or molasses-based fermentation by wild type c. acetobutylicum. molar balance analysis for improved process of mixed sugars utilization also revealed less acid accumulation and more butanol yield by the engineered strain as compared to the wild type. this study offers a genetic modification strategy for improving simultaneous utilization of mixed sugars by clostridium, which is essential for commercial exploitation of lignocellulose for the production of solvents and biofuels. (c) 2010 elsevier inc. all rights reserved. |
| WOS关键词 | CONTROL PROTEIN CCPA ; BACILLUS-SUBTILIS ; CATABOLITE REPRESSION ; GENE-EXPRESSION ; BUTANOL PRODUCTION ; ESCHERICHIA-COLI ; ATCC-824 ; FERMENTATION ; OPERON ; GROWTH |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000281074900004 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2407930 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, Sheng |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Shanghai Res & Dev Ctr Ind Biotechnol, Shanghai 201201, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Cong,Gu, Yang,Hu, Shiyuan,et al. Identification and inactivation of pleiotropic regulator ccpa to eliminate glucose repression of xylose utilization in clostridium acetobutylicum[J]. Metabolic engineering,2010,12(5):446-454. |
| APA | Ren, Cong.,Gu, Yang.,Hu, Shiyuan.,Wu, Yan.,Wang, Pan.,...&Jiang, Weihong.(2010).Identification and inactivation of pleiotropic regulator ccpa to eliminate glucose repression of xylose utilization in clostridium acetobutylicum.Metabolic engineering,12(5),446-454. |
| MLA | Ren, Cong,et al."Identification and inactivation of pleiotropic regulator ccpa to eliminate glucose repression of xylose utilization in clostridium acetobutylicum".Metabolic engineering 12.5(2010):446-454. |
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来源：中国科学院大学
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