中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
The rice light-regulated gene ra68 encodes a novel protein interacting with oxygen-evolving complex psbo mature protein

文献类型:期刊论文

作者Li, Tang1,2; Gong, Chunyan1,2; Wang, Tai1
刊名Plant molecular biology reporter
出版日期2010-03-01
卷号28期号:1页码:136-143
关键词Light-regulated gene Oryza sativa Oxygen-evolving complex Psbo Ra68 Protein interaction
ISSN号0735-9640
DOI10.1007/s11105-009-0128-x
通讯作者Wang, tai(twang@ibcas.ac.cn)
英文摘要The oxygen-evolving complex (oec), which is located on the luminal side of photosystem ii, plays an important role in water oxidation. it is generally considered that oec consists of the mn(4)ca cluster and three extrinsic proteins, psbo, psbp, and psbq. in this study, we report that a novel rice protein ra68 interacts with psbo. ra68 is expressed preferentially in seedlings and encodes a novel protein without significant homology with any other proteins. northern analysis demonstrates that ra68 is a light-regulated gene with a diurnal oscillation pattern under different light conditions. yeast two-hybrid screening reveals that ra68 interacts with psbo and psbp. further experiments demonstrate that ra68 has specific interaction with psbo mature protein rather than its precursor form. moreover, in situ hybridization shows that ra68 and psbo have similar expression patterns in seedlings.
WOS关键词MOLECULAR-MASS SUBUNITS ; PHOTOSYSTEM-II ; EXTRINSIC PROTEINS ; SIGNAL PEPTIDES ; NUCLEAR GENES ; HIGHER-PLANTS ; ELEMENTS ; BINDING ; POLYPEPTIDES ; EXPRESSION
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
WOS类目Biochemical Research Methods ; Plant Sciences
语种英语
WOS记录号WOS:000274249700015
出版者SPRINGER
URI标识http://www.irgrid.ac.cn/handle/1471x/2408004
专题中国科学院大学
通讯作者Wang, Tai
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Photosynthesis & Environm Mol Physiol, Res Ctr Mol & Dev Biol, Beijing 100093, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Li, Tang,Gong, Chunyan,Wang, Tai. The rice light-regulated gene ra68 encodes a novel protein interacting with oxygen-evolving complex psbo mature protein[J]. Plant molecular biology reporter,2010,28(1):136-143.
APA Li, Tang,Gong, Chunyan,&Wang, Tai.(2010).The rice light-regulated gene ra68 encodes a novel protein interacting with oxygen-evolving complex psbo mature protein.Plant molecular biology reporter,28(1),136-143.
MLA Li, Tang,et al."The rice light-regulated gene ra68 encodes a novel protein interacting with oxygen-evolving complex psbo mature protein".Plant molecular biology reporter 28.1(2010):136-143.

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来源:中国科学院大学

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