Magnetic nanoparticle-linked colorimetric aptasensor for the detection of thrombin
文献类型：期刊论文
| 作者 | Zhang, Zhanxia1,2; Wang, Zhongjun1,2; Wang, Xiaolei1,2; Yang, Xiurong1 |
| 刊名 | Sensors and actuators b-chemical
 |
| 出版日期 | 2010-06-03 |
| 卷号 | 147期号:2页码:428-433 |
| 关键词 | Magnetic nanoparticles Peroxidase mimic Aptamer Thrombin Colorimetry assay |
| ISSN号 | 0925-4005 |
| DOI | 10.1016/j.snb.2010.02.013 |
| 通讯作者 | Yang, xiurong(xryang@ciac.jl.cn) |
| 英文摘要 | Employed as a peroxidase mimic, chitosan modified fe(3)o(4) magnetic nanoparticles (mnps) were synthesized via solvotherrnal method. this alternative model evidenced a remarkable catalytic activity in presence of hydrogen peroxide/3,3',5,5'-tetramethylbenzidine (tmb) solution. fe(3)o(4) peroxidase mimic has the merits of stability, magnetic separation and enrichment. enzyme-linked immunosorbent assay (elisa) was used to detect thrombin by employing the catalytic properties of the as-synthesized chitosan modified fe(3)o(4) mnps. the results showed that the absorption values at 652 nm increased with the thrombin concentrations, a linear range from 1 to 100 nm and 1 nm thrombin (s/n = 3) could be detected. the proposed approach confirmed that the fe(3)o(4) mnps not only possessed peroxidase activity but also showed potential application in varieties of simple, robust, and cost-effective analytical methods in the future. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | GOLD NANOPARTICLES ; DNA APTAMERS ; BIOSENSOR ; ELECTRODE ; CHITOSAN ; LIGANDS ; ASSAY ; FTIR |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000278679100008 |
| 出版者 | ELSEVIER SCIENCE SA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2408032 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, Xiurong |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhanxia,Wang, Zhongjun,Wang, Xiaolei,et al. Magnetic nanoparticle-linked colorimetric aptasensor for the detection of thrombin[J]. Sensors and actuators b-chemical,2010,147(2):428-433. |
| APA | Zhang, Zhanxia,Wang, Zhongjun,Wang, Xiaolei,&Yang, Xiurong.(2010).Magnetic nanoparticle-linked colorimetric aptasensor for the detection of thrombin.Sensors and actuators b-chemical,147(2),428-433. |
| MLA | Zhang, Zhanxia,et al."Magnetic nanoparticle-linked colorimetric aptasensor for the detection of thrombin".Sensors and actuators b-chemical 147.2(2010):428-433. |
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来源：中国科学院大学
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