Efficient derivation of chinese human embryonic stem cell lines from frozen embryos
文献类型：期刊论文
| 作者 | Li, Chunliang2,5; Yang, Ying2,5; Lu, Xiaowei4; Sun, Yijuan4; Gu, Junjie2,3; Feng, Yun4; Jin, Ying1,2,3 |
| 刊名 | In vitro cellular & developmental biology-animal
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 46期号:3-4页码:186-191 |
| 关键词 | Human embryonic stem cells Derivation Characterization |
| ISSN号 | 1071-2690 |
| DOI | 10.1007/s11626-010-9304-4 |
| 通讯作者 | Jin, ying(yjin@sibs.ac.cn) |
| 英文摘要 | Human embryonic stem (hes) cells are pluripotent cells derived from the inner cell mass of blastocysts. their unique properties of self-renewal and pluripotency make them an attractive tool for basic research as well as a potential cell resource for therapy. however, each hes cell line demonstrates different identity. it is desirable to obtain more fully characterized hes cell lines with newly developed technologies associated with hes cell culture. here, we report our experience of efficient derivation of three new chinese hes cell lines (shhes2, shhes3, and shhes4) from in vitro fertilization discarded embryos donated by women with polycystic ovary syndrome. these cell lines were derived under conditions minimizing exposure to animal components and maintained at an undifferentiated state for long-term culture. they retained a normal karyotype and expressed alp, oct4, sox2, ssea-4, tra-1-60 and tra-1-81. rt-pcr analysis also revealed high expression levels of pluripotency markers such as oct4, lefty a, sox2, tdgf-1, thy1, fgf4, nanog, and rex1. when suspended in low-attachment culture dishes, embryoid bodies formed and were comprised of various differentiated cell types from all three embryonic germ layers. however, well-shaped teratomas were only harvested from line shhes2, not from shhes3 and shhes4, indicating that the differentiation ability in vivo differs among the three cell lines. collectively, the three new hes cell lines were established and fully characterized. the effort paves the way toward generating hes cell lines without contamination by animal components. all of these cell lines are available by contact ying jin at yjin@sibs.ac.cn. |
| WOS关键词 | POLYCYSTIC-OVARY-SYNDROME |
| WOS研究方向 | Cell Biology ; Developmental Biology |
| WOS类目 | Cell Biology ; Developmental Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000276768700005 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2408262 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jin, Ying |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Key Lab Cell Differentiat & Apoptosis, Chinese Minist Educ, Shanghai 200025, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Key Lab Stem Cell Biol, Shanghai Inst Biol Sci,Inst Hlth Sci,Chinese Acad, Shanghai 200025, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Med, Shanghai Inst Stem Cell Res, Shanghai 200025, Peoples R China 4.Ruijin Hosp, Reprod Med Ctr, Shanghai 200135, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chunliang,Yang, Ying,Lu, Xiaowei,et al. Efficient derivation of chinese human embryonic stem cell lines from frozen embryos[J]. In vitro cellular & developmental biology-animal,2010,46(3-4):186-191. |
| APA | Li, Chunliang.,Yang, Ying.,Lu, Xiaowei.,Sun, Yijuan.,Gu, Junjie.,...&Jin, Ying.(2010).Efficient derivation of chinese human embryonic stem cell lines from frozen embryos.In vitro cellular & developmental biology-animal,46(3-4),186-191. |
| MLA | Li, Chunliang,et al."Efficient derivation of chinese human embryonic stem cell lines from frozen embryos".In vitro cellular & developmental biology-animal 46.3-4(2010):186-191. |
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来源：中国科学院大学
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