Nuclear transfer-derived epiblast stem cells are transcriptionally and epigenetically distinguishable from their fertilized-derived counterparts
文献类型：期刊论文
| 作者 | Maruotti, Julien1,2,3,4; Dai, Xiang Peng5; Brochard, Vincent1,4; Jouneau, Luc1,4; Liu, Jun6; Bonnet-Garnier, Amelie1,4; Jammes, Helene1,4; Vallier, Ludovic7; Brons, I. Gabrielle M.7; Pedersen, Roger7 |
| 刊名 | Stem cells
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 28期号:4页码:743-752 |
| 关键词 | Reprogramming Nuclear transfer Cloning Pluripotent stem cells |
| ISSN号 | 1066-5099 |
| DOI | 10.1002/stem.400 |
| 通讯作者 | Jouneau, alice(alice.jouneau@jouy.inra.fr) |
| 英文摘要 | Mouse embryonic pluripotent stem cells can be obtained from the inner cell mass at the blastocyst stage (embryonic stem cells, escs) or from the late epiblast of postimplantation embryos (epiblast stem cells, episcs). during normal development, the transition between these two stages is marked by major epigenetic and transcriptional changes including dna de novo methylation. these modifications represent an epigenetic mark conserved in escs and episcs. pluripotent escs derived from blastocysts generated by nuclear transfer (nt) have been shown to be correctly reprogrammed. however, nt embryos frequently undergo abnormal development. in the present study, we have examined whether pluripotent cells could be derived from the epiblast of postimplantation nt embryos and whether the reprogramming process would affect the epigenetic changes occurring at this stage, which could explain abnormal development of nt embryos. we showed that episcs could be derived with the same efficiency from nt embryos and from their fertilized counterparts. however, gene expression profile analyses showed divergence between fertilized-and nuclear transfer-episcs with a surprising bias in the distribution of the differentially expressed genes, 30% of them being localized on chromosome 11. a majority of these genes were downregulated in nt-episcs and imprinted genes represented a significant fraction of them. notably, analysis of the epigenetic status of a downregulated imprinted gene in nt-episcs revealed complete methylation of the two alleles. therefore, episcs derived from nt embryos appear to be incorrectly reprogrammed, indicating that abnormal epigenetic marks are imposed on cells in nt embryos during the transition from early to late epiblast. stem cells 2010;28:743-752 |
| WOS关键词 | PRADER-WILLI-SYNDROME ; IMPRINTED GENE METHYLATION ; CLONED MOUSE EMBRYOS ; DNA METHYLATION ; ES CELLS ; MATERNAL-BEHAVIOR ; EXPRESSION ; PLURIPOTENT ; NECDIN ; MICE |
| WOS研究方向 | Cell Biology ; Biotechnology & Applied Microbiology ; Oncology ; Hematology |
| WOS类目 | Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Oncology ; Cell Biology ; Hematology |
| 语种 | 英语 |
| WOS记录号 | WOS:000277095400013 |
| 出版者 | ALPHAMED PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2408753 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jouneau, Alice |
| 作者单位 | 1.INRA, UMR Biol Dev & Reprod 1198, Jouy En Josas, France 2.Chinese Acad Sci, Inst Zool, Sinofrench Lab LABIOCEM, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.ENVA, Maisons Alfort, France 5.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 6.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China 7.Univ Cambridge, Lab Regenerat Med, Dept Surg, Cambridge, England |
| 推荐引用方式 GB/T 7714 | Maruotti, Julien,Dai, Xiang Peng,Brochard, Vincent,et al. Nuclear transfer-derived epiblast stem cells are transcriptionally and epigenetically distinguishable from their fertilized-derived counterparts[J]. Stem cells,2010,28(4):743-752. |
| APA | Maruotti, Julien.,Dai, Xiang Peng.,Brochard, Vincent.,Jouneau, Luc.,Liu, Jun.,...&Jouneau, Alice.(2010).Nuclear transfer-derived epiblast stem cells are transcriptionally and epigenetically distinguishable from their fertilized-derived counterparts.Stem cells,28(4),743-752. |
| MLA | Maruotti, Julien,et al."Nuclear transfer-derived epiblast stem cells are transcriptionally and epigenetically distinguishable from their fertilized-derived counterparts".Stem cells 28.4(2010):743-752. |
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来源：中国科学院大学
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