Identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella)
文献类型：期刊论文
| 作者 | Liu, Feng2; Wang, Dapeng1,3; Fu, Jianjun2; Sun, Gaoyuan1; Shen, Yubang2; Dong, Lingli1; Zhang, Bing1; Hu, Songnian1; Li, Jiale2,4 |
| 刊名 | Comparative biochemistry and physiology d-genomics & proteomics
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 5期号:2页码:116-123 |
| 关键词 | Grass carp Cdna library Head kidney Immune-related gene Fcr gamma Immunoglobulin light chains |
| ISSN号 | 1744-117X |
| DOI | 10.1016/j.cbd.2010.02.003 |
| 通讯作者 | Hu, songnian(husn@big.ac.cn) |
| 英文摘要 | Grass carp is the third largest aquaculture species in global production. however, genomic research of this species has been limited. to identify immune-related genes in grass carp, a normalized full-length cdna library was constructed from head kidney tissues, and 6432 randomly selected clones were sequenced. 5289 high quality expressed sequence tags (est) were generated and assembled into 2687 unigenes. among them, 1585 unigenes showed significant similarity with known sequences in public databases, whereas the remaining 1102 unigenes appeared to be novel sequences with unknown functions. in particular, 136 immune-related genes were identified to encode immunoglobulins, fcr gamma, ifn-related proteins, various cd markers, mhcs, complements and other important immune-related factors: a majority of these genes are reported in grass carp for the first time. sequence analysis indicated that grass carp has at least three subtypes of immunoglobulin light chains, namely l1, l2 and l3. furthermore, fcr gamma was found to broadly express in different tissues. our study constitutes the first est analysis of lymphatic tissue in grass carp, and could pave the way for further research of immune-related genes and functional genomics in grass carp. crown copyright (c) 2010 published by elsevier inc. all rights reserved. |
| WOS关键词 | LIGHT-CHAIN ISOTYPES ; CDNA LIBRARY ; GENOMIC ORGANIZATION ; MOLECULAR-CLONING ; CHANNEL CATFISH ; COMMON CARP ; IMMUNOGLOBULIN ; VERTEBRATES ; SYSTEM ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS类目 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000279214000005 |
| 出版者 | ELSEVIER SCIENCE INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2408816 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Hu, Songnian |
| 作者单位 | 1.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genome Sci & Informat, Beijing 100029, Peoples R China 2.Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai 201306, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Shanghai Ocean Univ, E Inst Shanghai Univ, Aquaculture Div, Shanghai 201306, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Feng,Wang, Dapeng,Fu, Jianjun,et al. Identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella)[J]. Comparative biochemistry and physiology d-genomics & proteomics,2010,5(2):116-123. |
| APA | Liu, Feng.,Wang, Dapeng.,Fu, Jianjun.,Sun, Gaoyuan.,Shen, Yubang.,...&Li, Jiale.(2010).Identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella).Comparative biochemistry and physiology d-genomics & proteomics,5(2),116-123. |
| MLA | Liu, Feng,et al."Identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella)".Comparative biochemistry and physiology d-genomics & proteomics 5.2(2010):116-123. |
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来源：中国科学院大学
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