Arabidopsis villin5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth
文献类型：期刊论文
| 作者 | Zhang, Hua1,2; Qu, Xiaolu1,2; Bao, Chanchan1,2; Khurana, Parul3,4; Wang, Qiannan1,2; Xie, Yurong1; Zheng, Yiyan1,2; Chen, Naizhi1; Blanchoin, Laurent5; Staiger, Christopher J.3,4 |
| 刊名 | Plant cell
 |
| 出版日期 | 2010-08-01 |
| 卷号 | 22期号:8页码:2749-2767 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.110.076257 |
| 通讯作者 | Huang, shanjin(sjhuang@ibcas.ac.cn) |
| 英文摘要 | A dynamic actin cytoskeleton is essential for pollen germination and tube growth. however, the molecular mechanisms underlying the organization and turnover of the actin cytoskeleton in pollen remain poorly understood. villin plays a key role in the formation of higher-order structures from actin filaments and in the regulation of actin dynamics in eukaryotic cells. it belongs to the villin/gelsolin/fragmin superfamily of actin binding proteins and is composed of six gelsolin-homology domains at its core and a villin headpiece domain at its c terminus. recently, several villin family members from plants have been shown to sever, cap, and bundle actin filaments in vitro. here, we characterized a villin isovariant, arabidopsis thaliana villin5 (vln5), that is highly and preferentially expressed in pollen. vln5 loss-of-function retarded pollen tube growth and sensitized actin filaments in pollen grains and tubes to latrunculin b. in vitro biochemical analyses revealed that vln5 is a typical member of the villin family and retains a full suite of activities, including barbed-end capping, filament bundling, and calcium-dependent severing. the severing activity was confirmed with time-lapse evanescent wave microscopy of individual actin filaments in vitro. we propose that vln5 is a major regulator of actin filament stability and turnover that functions in concert with oscillatory calcium gradients in pollen and therefore plays an integral role in pollen germination and tube growth. |
| WOS关键词 | BINDING-PROTEIN ; PLANT VILLIN ; CELL-GROWTH ; SUPERFAMILY PROTEINS ; DROSOPHILA QUAIL ; POLARIZED GROWTH ; CAPPING PROTEIN ; ARP2/3 COMPLEX ; BRUSH-BORDERS ; IN-VIVO |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000282270600018 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2408998 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Huang, Shanjin |
| 作者单位 | 1.Chinese Acad Sci, Ctr Signal Transduct & Metabol, Key Lab Photosynthesis & Environm Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA 4.Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA 5.Univ Grenoble 1, Inst Rech Technol & Sci Vivant, Lab Physiol Cellulaire Vegetale, Commissariat Energie Atom,CNRS, F-38054 Grenoble, France |
| 推荐引用方式 GB/T 7714 | Zhang, Hua,Qu, Xiaolu,Bao, Chanchan,et al. Arabidopsis villin5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth[J]. Plant cell,2010,22(8):2749-2767. |
| APA | Zhang, Hua.,Qu, Xiaolu.,Bao, Chanchan.,Khurana, Parul.,Wang, Qiannan.,...&Huang, Shanjin.(2010).Arabidopsis villin5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth.Plant cell,22(8),2749-2767. |
| MLA | Zhang, Hua,et al."Arabidopsis villin5, an actin filament bundling and severing protein, is necessary for normal pollen tube growth".Plant cell 22.8(2010):2749-2767. |
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来源：中国科学院大学
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