Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies
文献类型：期刊论文
| 作者 | Li BX(李宝霞) ; Chen J(陈娟); Long M(龙勉)
|
| 刊名 | Chinese Science Bulletin/中国科学通报(英文版)
 |
| 出版日期 | 2008 |
| 页码 | 3634-3641 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| ISSN号 | 1001-6538 |
| 关键词 | Biacore Biosensor Response Unit Cellular Kinetic Model Cellular Off-Rate Cellular On-Rate Plasmon Resonance Listeria-Monocytogenes Mass-Transport Rate Constants Monoclonal-Antibody Optical Biosensor Small Systems Affinity Equilibrium Technology |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China. |
| 中文摘要 | Cell adhesion is crucial to many biological processes, such as inflammatory responses, tumor metastasis and thrombosis formation. Recently a commercial surface plasmon resonance (SPR)-based BIAcore biosensor has been extended to determine cell binding mediated by surface-bound biomolecular interactions. How such cell binding is quantitatively governed by kinetic rates and regulating factors, however, has been poorly understood. Here we developed a novel assay to determine the binding kinetics of surface-bound biomolecular interactions using a commercial BIAcore 3000 biosensor. Human red blood cells (RBCs) presenting blood group B antigen and CM5 chip bearing immobilized anti-B monoclonal antibody (mAb) were used to obtain the time courses of response unit, or sensorgrams, when flowing RBCs over the chip surface. A cellular kinetic model was proposed to correlate the sensorgrams with kinetic rates. Impacts of regulating factors, such as cell concentration, flow duration and rate, antibody-presenting level, as well as pH value and osmotic pressure of suspending medium were tested systematically, which imparted the confidence that the approach can be applied to kinetic measurements of cell adhesion mediated by surface-bound biomolecular interactions. These results provided a new insight into quantifying cell binding using a commercial SPR-based BIAcore biosensor. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | PLASMON RESONANCE ; LISTERIA-MONOCYTOGENES ; MASS-TRANSPORT ; RATE CONSTANTS ; MONOCLONAL-ANTIBODY ; OPTICAL BIOSENSOR ; SMALL SYSTEMS ; AFFINITY ; EQUILIBRIUM ; TECHNOLOGY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261257600005 |
| 公开日期 | 2009-08-03 ; 2009-09-14 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/25818]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Li BX,Chen J,Long M. Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies[J]. Chinese Science Bulletin/中国科学通报(英文版),2008:3634-3641. |
| APA | 李宝霞,陈娟,&龙勉.(2008).Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies.Chinese Science Bulletin/中国科学通报(英文版),3634-3641. |
| MLA | 李宝霞,et al."Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies".Chinese Science Bulletin/中国科学通报(英文版) (2008):3634-3641. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。