Small ubiquitin-related modifier paralogs are indispensable but functionally redundant during early development of zebrafish
文献类型：期刊论文
| 作者 | Yuan, Hao1,2,3,4; Zhou, Jun1; Deng, Min3,4,5; Liu, Xi1; Le Bras, Morgane; de The, Hugues1,6; Chen, Sai Juan1,2,3,4; Chen, Zhu1,2,3,4; Liu, Ting Xi3,4,5; Zhu, Jun1 |
| 刊名 | Cell research
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 20期号:2页码:185-196 |
| 关键词 | Sumo paralogs Zebrafish P53 |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2009.101 |
| 通讯作者 | Zhu, jun(jzhu1966@yahoo.com.cn) |
| 英文摘要 | The small ubiquitin-related modifier (sumo) conjugation to a variety of proteins regulates diverse cellular processes, including transcription, cell cycle regulation and maintenance of genome integrity. to investigate in vivo biological function of sumo paralogs, we inactivated them in the early development of zebrafish. while zebrafish embryos deficient for all three sumo paralogs, as ubc9-deficient ones, displayed severe defects, loss of individual sumo paralog was compatible with a normal development. sumo-deficient embryos can be rescued by a single human or zebrafish sumo. while key structural basic lysine residues and n-terminal unstructured stretch of sumo are critical for in vivo rescue, the consensus k11 sumoylation site of sumo2 is dispensable, implying that chain formation on this potential site is unessential for normal development. inactivation of all three sumos triggered p53-dependent apoptosis and further inactivation of p53 restored normal zebrafish development. interestingly, we also demonstrate that the dominant negative truncated form of p53, delta 113p53, significantly blunts sumo depletion-induced p53 activity in vivo. taken together, our results suggest that sumo paralogs are indispensable, but redundant, in the early development of zebrafish. |
| WOS关键词 | CHROMOSOME SEGREGATION ; TRANSCRIPTION FACTORS ; SUMO-1 MODIFICATION ; CONJUGATING ENZYME ; MOUSE DEVELOPMENT ; HOMEOBOX GENE ; SUMOYLATION ; ALPHA ; PML ; P53 |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000275816100010 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2409035 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhu, Jun |
| 作者单位 | 1.Rui Jin Hosp, CNRS LIA, Shanghai Inst Hematol, Shanghai 200025, Peoples R China 2.Chinese Acad Sci, State Key Lab Med Genom, Inst Hlth Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China 3.Chinese Acad Sci, Grad Sch, Shanghai 200025, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China 5.Chinese Acad Sci, Lab Dev & Dis, Inst Hlth Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China 6.Hop St Louis, CNRS, UMR 7151, F-75475 Paris 10, France |
| 推荐引用方式 GB/T 7714 | Yuan, Hao,Zhou, Jun,Deng, Min,et al. Small ubiquitin-related modifier paralogs are indispensable but functionally redundant during early development of zebrafish[J]. Cell research,2010,20(2):185-196. |
| APA | Yuan, Hao.,Zhou, Jun.,Deng, Min.,Liu, Xi.,Le Bras, Morgane.,...&Zhu, Jun.(2010).Small ubiquitin-related modifier paralogs are indispensable but functionally redundant during early development of zebrafish.Cell research,20(2),185-196. |
| MLA | Yuan, Hao,et al."Small ubiquitin-related modifier paralogs are indispensable but functionally redundant during early development of zebrafish".Cell research 20.2(2010):185-196. |
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来源：中国科学院大学
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