Reversible association of ribulose-1, 5-bisphosphate carboxylase/oxygenase activase with the thylakoid membrane depends upon the atp level and ph in rice without heat stress
文献类型：期刊论文
| 作者 | Chen, Juan; Wang, Peng; Mi, Hua-ling; Chen, Gen-Yun; Xu, Da-Quan |
| 刊名 | Journal of experimental botany
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 61期号:11页码:2939-2950 |
| 关键词 | Activation Atp Delta ph Rubisco Rubisco activase Thylakoid membrane |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/erq122 |
| 通讯作者 | Chen, gen-yun(chenggy@sippe.ac.cn) |
| 英文摘要 | Ribulose-1, 5-bisphosphate carboxylase/oxygenase (rubisco) activase (rca) in the thylakoid membrane (tm) has been shown to play a role in protection and regulation of photosynthesis under moderate heat stress. however, the physiological significance of rca bound to the tm (tm-rca) without heat stress remains unknown. in this study, it is first shown, using experiments in vivo, that the tm-rca varies in rice leaves at different development stages, under different environmental conditions, and in a rice mutant. furthermore, it is shown that the amount of tm-rca always increased when the rubisco activation state and the ph gradient across the tm (delta ph) decreased. it was then demonstrated in vitro that the rca bound dynamically to tm and the amount of tm-rca increased during rubisco activation. a high level of atp and a high ph value promoted the dissociation of rca from the tm. both the rca association with and dissociation from the tm showed conformational changes related to the atp level or ph as indicated by the changes in fluorescence intensity of 1-anilinonaphthalene-8-sulphonic acid (ans) binding to rca. these results suggest that the reversible association of rca with the tm is atp and ph (or delta ph) dependent; it might be involved in the rca activation of rubisco, in addition to the previously discovered role in the protection and regulation of photosynthesis under heat stress. |
| WOS关键词 | ARABIDOPSIS-RUBISCO ACTIVASE ; HIGH-TEMPERATURE STRESS ; CHLOROPHYLL FLUORESCENCE ; ELECTRON-TRANSPORT ; BISPHOSPHATE CARBOXYLASE ; ENHANCES PHOTOSYNTHESIS ; OXYGENASE ACTIVASE ; PROTEIN COMPLEXES ; LIGHT ACTIVATION ; PHOTOSYSTEM-I |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000279188100007 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2409680 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Gen-Yun |
| 作者单位 | Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Grad Sch, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Juan,Wang, Peng,Mi, Hua-ling,et al. Reversible association of ribulose-1, 5-bisphosphate carboxylase/oxygenase activase with the thylakoid membrane depends upon the atp level and ph in rice without heat stress[J]. Journal of experimental botany,2010,61(11):2939-2950. |
| APA | Chen, Juan,Wang, Peng,Mi, Hua-ling,Chen, Gen-Yun,&Xu, Da-Quan.(2010).Reversible association of ribulose-1, 5-bisphosphate carboxylase/oxygenase activase with the thylakoid membrane depends upon the atp level and ph in rice without heat stress.Journal of experimental botany,61(11),2939-2950. |
| MLA | Chen, Juan,et al."Reversible association of ribulose-1, 5-bisphosphate carboxylase/oxygenase activase with the thylakoid membrane depends upon the atp level and ph in rice without heat stress".Journal of experimental botany 61.11(2010):2939-2950. |
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来源：中国科学院大学
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