Quantitative proteomic analysis of s-nitrosated proteins in diabetic mouse liver with icat switch method
文献类型：期刊论文
| 作者 | Zhang, Xu1,2; Huang, Bo1,2; Zhou, Xixi1,2; Chen, Chang1 |
| 刊名 | Protein & cell
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 1期号:7页码:675-687 |
| 关键词 | Icat switch Mass spectrometry Quantitative S-nitrosation Type 2 diabetes |
| ISSN号 | 1674-800X |
| DOI | 10.1007/s13238-010-0087-x |
| 通讯作者 | Chen, chang(changchen@moon.ibp.ac.cn) |
| 英文摘要 | In this study we developed a quantitative proteomic method named icatswitch by introducing isotope-coded affinity tag (icat) reagents into the biotin-switch method, and used it to investigate s-nitrosation in the liver of normal control c57bl/6j mice and type 2 diabetic kk-ay mice. we got fifty-eight s-nitrosated peptides with quantitative information in our research, among which thirty-seven had changed s-nitrosation levels in diabetic mouse liver. the s-nitrosated peptides belonged to forty-eight proteins (twenty-eight were new s-nitrosated proteins), some of which were new targets of s-nitrosation and known to be related with diabetes. s-nitrosation patterns were different between diabetic and normal mice. gene ontology enrichment results suggested that s-nitrosated proteins are more abundant in amino acid metabolic processes. the network constructed for s-nitrosated proteins by text-mining technology provided clues about the relationship between s-nitrosation and type 2 diabetes. our work provides a new approach for quantifying s-nitrosated proteins and suggests that the integrative functions of s-nitrosation may take part in pathophysiological processes of type 2 diabetes. |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000208511100010 |
| 出版者 | HIGHER EDUCATION PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2409921 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chen, Chang |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xu,Huang, Bo,Zhou, Xixi,et al. Quantitative proteomic analysis of s-nitrosated proteins in diabetic mouse liver with icat switch method[J]. Protein & cell,2010,1(7):675-687. |
| APA | Zhang, Xu,Huang, Bo,Zhou, Xixi,&Chen, Chang.(2010).Quantitative proteomic analysis of s-nitrosated proteins in diabetic mouse liver with icat switch method.Protein & cell,1(7),675-687. |
| MLA | Zhang, Xu,et al."Quantitative proteomic analysis of s-nitrosated proteins in diabetic mouse liver with icat switch method".Protein & cell 1.7(2010):675-687. |
入库方式： iSwitch采集
来源：中国科学院大学
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