A simple hairpin dna sensor for label-free detection of sub-attomole dna target
文献类型：期刊论文
| 作者 | Luan, Qingfen1; Xue, Ying1; Yao, Xin1,2 |
| 刊名 | Sensors and actuators b-chemical
 |
| 出版日期 | 2010-06-03 |
| 卷号 | 147期号:2页码:561-565 |
| ISSN号 | 0925-4005 |
| 关键词 | Hairpin dna probe Surface plasmon resonance Dna sensor Label-free Ultrasensitive |
| DOI | 10.1016/j.snb.2010.03.059 |
| 通讯作者 | Yao, xin(yaox@gucas.ac.cn) |
| 英文摘要 | In this paper, we explored a label-free, ultrasensitive and simple method of dna detection based on hairpin dna via a high-resolution surface plasmon resonance (spr). using this simple direct hybridization system, a detection level of 0.5 fm was obtained and the reason of the high sensitivity was discussed. moreover, the prepared dna sensor could also discriminate single-base mismatched target and was amenable to study the effect of mismatch position in target dna. from the results, it can be seen that mutation in the center of target dna had a greater influence on the hybridization. then, a simple, inexpensive, high sensitive and excellent selective dna sensor was developed. (c) 2010 elsevier b.v. all rights reserved. |
| WOS关键词 | SURFACE-PLASMON RESONANCE ; MOLECULAR BEACON BIOSENSORS ; SEQUENCE-SPECIFIC DETECTION ; SELF-ASSEMBLED MONOLAYERS ; ELECTROCHEMICAL DETECTION ; ULTRASENSITIVE DETECTION ; HYBRIDIZATION ; OLIGONUCLEOTIDE ; PROBES ; GOLD |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE SA |
| WOS记录号 | WOS:000278679100027 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2409951 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yao, Xin |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Coll Chem & Chem Engn, Beijing 100049, Peoples R China 2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luan, Qingfen,Xue, Ying,Yao, Xin. A simple hairpin dna sensor for label-free detection of sub-attomole dna target[J]. Sensors and actuators b-chemical,2010,147(2):561-565. |
| APA | Luan, Qingfen,Xue, Ying,&Yao, Xin.(2010).A simple hairpin dna sensor for label-free detection of sub-attomole dna target.Sensors and actuators b-chemical,147(2),561-565. |
| MLA | Luan, Qingfen,et al."A simple hairpin dna sensor for label-free detection of sub-attomole dna target".Sensors and actuators b-chemical 147.2(2010):561-565. |
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来源：中国科学院大学
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