Improvement of human interferon alpha secretion by lactococcus lactis
文献类型：期刊论文
| 作者 | Zhang, Qiuxiang1,2; Zhong, Jin1; Liang, Xiaobo2; Liu, Wenjun3; Huan, Liandong1 |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2010-09-01 |
| 卷号 | 32期号:9页码:1271-1277 |
| ISSN号 | 0141-5492 |
| 关键词 | Human interferon alpha Lactococcus lactis Leisstcda Secretion |
| DOI | 10.1007/s10529-010-0285-x |
| 通讯作者 | Zhong, jin(zhongj@im.ac.cn) |
| 英文摘要 | To improve the secretion and expression of human interferon alpha 2b (ifn) in lactococcus lactis, a synthetic pro-peptide, leisstcda (leiss), was fused to the n-terminus of ifn. this gave a higher secretion efficiency (12% vs. 5%) and yield (similar to 2.8-fold) of ifn. the signal peptide, spslpa (slpa, an s-layer protein of lactobacillus brevis), was also tested to secrete ifn instead of spusp45 (usp45, the main secreted protein in l. lactis). this gave increased ifn secretion (similar to 3-fold) but lower total production. all the recombinant ifn had appropriate bioactivities in an antiviral assay. |
| WOS关键词 | ESCHERICHIA-COLI ; I INTERFERON ; EXPRESSION |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000280892800013 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2409995 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhong, Jin |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Ctr Mol Virol, Inst Microbiol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qiuxiang,Zhong, Jin,Liang, Xiaobo,et al. Improvement of human interferon alpha secretion by lactococcus lactis[J]. Biotechnology letters,2010,32(9):1271-1277. |
| APA | Zhang, Qiuxiang,Zhong, Jin,Liang, Xiaobo,Liu, Wenjun,&Huan, Liandong.(2010).Improvement of human interferon alpha secretion by lactococcus lactis.Biotechnology letters,32(9),1271-1277. |
| MLA | Zhang, Qiuxiang,et al."Improvement of human interferon alpha secretion by lactococcus lactis".Biotechnology letters 32.9(2010):1271-1277. |
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来源：中国科学院大学
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