Disruption of actin filaments induces mitochondrial ca2+ release to the cytoplasm and [ca2+](c) changes in arabidopsis root hairs
文献类型：期刊论文
| 作者 | Wang, Yuqing1,2,3; Zhu, Yingfang1,2; Ling, Yu1,2; Zhang, Haiyan1; Liu, Peng1,2; Baluska, Frantisek4; Samaj, Jozef5; Lin, Jinxing1; Wang, Qinli1 |
| 刊名 | Bmc plant biology
 |
| 出版日期 | 2010-03-24 |
| 卷号 | 10页码:13 |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/1471-2229-10-53 |
| 通讯作者 | Wang, qinli(wql@ibcas.ac.cn) |
| 英文摘要 | Background: mitochondria are dynamic organelles that move along actin filaments, and serve as calcium stores in plant cells. the positioning and dynamics of mitochondria depend on membrane-cytoskeleton interactions, but it is not clear whether microfilament cytoskeleton has a direct effect on mitochondrial function and ca2+ storage. therefore, we designed a series of experiments to clarify the effects of actin filaments on mitochondrial ca2+ storage, cytoplasmic ca2+ concentration ([ca2+](c)), and the interaction between mitochondrial ca2+ and cytoplasmic ca2+ in arabidopsis root hairs. results: in this study, we found that treatments with latrunculin b (lat-b) and jasplakinolide (jas), which depolymerize and polymerize actin filaments respectively, decreased membrane potential and ca2+ stores in the mitochondria of arabidopsis root hairs. simultaneously, these treatments induced an instantaneous increase of cytoplasmic ca2+, followed by a continuous decrease. all of these effects were inhibited by pretreatment with cyclosporin a (cs a), a representative blocker of the mitochondrial permeability transition pore (mptp). moreover, we found there was a ca2+ concentration gradient in mitochondria from the tip to the base of the root hair, and this gradient could be disrupted by actin-acting drugs. conclusions: based on these results, we concluded that the disruption of actin filaments caused by lat-b or jas promoted irreversible opening of the mptp, resulting in mitochondrial ca2+ release into the cytoplasm, and consequent changes in [ca2+](c). we suggest that normal polymerization and depolymerization of actin filaments are essential for mitochondrial ca2+ storage in root hairs. |
| WOS关键词 | PERMEABILITY TRANSITION PORE ; PROGRAMMED CELL-DEATH ; ENDOPLASMIC-RETICULUM ; PLASMA-MEMBRANE ; F-ACTIN ; CALCIUM-RELEASE ; SALT STRESS ; CYTOSKELETON ; PLANTS ; CHANNELS |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000278022700001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2410009 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Qinli |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Photosynth & Mol Environm Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China 4.Univ Bonn, Inst Cellular & Mol Bot, Dept Plant Cell Biol, D-53115 Bonn, Germany 5.Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, Fac Sci, Olomouc 78301, Czech Republic |
| 推荐引用方式 GB/T 7714 | Wang, Yuqing,Zhu, Yingfang,Ling, Yu,et al. Disruption of actin filaments induces mitochondrial ca2+ release to the cytoplasm and [ca2+](c) changes in arabidopsis root hairs[J]. Bmc plant biology,2010,10:13. |
| APA | Wang, Yuqing.,Zhu, Yingfang.,Ling, Yu.,Zhang, Haiyan.,Liu, Peng.,...&Wang, Qinli.(2010).Disruption of actin filaments induces mitochondrial ca2+ release to the cytoplasm and [ca2+](c) changes in arabidopsis root hairs.Bmc plant biology,10,13. |
| MLA | Wang, Yuqing,et al."Disruption of actin filaments induces mitochondrial ca2+ release to the cytoplasm and [ca2+](c) changes in arabidopsis root hairs".Bmc plant biology 10(2010):13. |
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来源：中国科学院大学
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