Prostasin regulates human placental trophoblast cell proliferation via the epidermal growth factor receptor signaling pathway
文献类型：期刊论文
| 作者 | Fu, Ya-Yuan1,2; Gao, Wen-Long1,2; Chen, Mengqian3,4; Chai, Karl X.3,4; Wang, Yan-Ling1; Chen, Li-Mei3,4 |
| 刊名 | Human reproduction
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 25期号:3页码:623-632 |
| 关键词 | Prostasin Trophoblast Cell proliferation Epidermal growth factor receptor-mitogen-activated protein kinase signaling |
| ISSN号 | 0268-1161 |
| DOI | 10.1093/humrep/dep457 |
| 通讯作者 | Wang, yan-ling(wangyl@ioz.ac.cn) |
| 英文摘要 | Prostasin is a glycosylphosphatidylinositol-anchored extracellular serine protease with a role in epidermal growth factor receptor (egfr) signal modulation. egfr signaling has been shown to be important for regulating cytotrophoblast (ct) cell proliferation in human placenta. we investigated the impact of prostasin expression regulation on this cellular function as well as the molecular mechanisms involved in human cytotrophoblastic cells. an immortalized normal human ct cell line (b6tert-1) was used as an in vitro cell model. prostasin expression in b6tert-1 cells was knocked down by transfection of a short interfering rna. lentivirus-mediated expression of recombinant human prostasin under tetracycline regulation was performed to obtain stable b6tert-1 cell sublines that over-expressed prostasin. changes in cell proliferation and egfr signaling were evaluated by immunocytochemistry for ki67 and western blot analysis, respectively, in b6tert-1 cells with knocked-down or increased prostasin expression. prostasin knock-down in b6tert-1 cells resulted in inhibition of cell proliferation, in association with down-regulated egfr protein expression (both p < 0.05 versus control) as well as reduced phosphorylation of c-raf, mitogen-activated protein kinase (mapk) kinases (mek1/2) and extracellular signal-regulated kinases (erk1/2) (all p < 0.05 versus control). over-expression of prostasin led to up-regulation of the egfr protein, but had no effect on cell proliferation or phosphorylation of mapk signaling molecules in the b6tert-1 cells. prostasin may regulate trophoblast cell proliferation via modulating the egfr-mapk signaling pathway. |
| WOS关键词 | SERINE-PROTEASE ; SODIUM-CHANNEL ; EXPRESSION ; EGFR ; CANCER ; LINE ; INVASIVENESS ; RETARDATION ; PREGNANCIES ; ACTIVATION |
| WOS研究方向 | Obstetrics & Gynecology ; Reproductive Biology |
| WOS类目 | Obstetrics & Gynecology ; Reproductive Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000274490700011 |
| 出版者 | OXFORD UNIV PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2410103 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Yan-Ling |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, Biomol Sci Ctr, Orlando, FL 32816 USA 4.Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, Dept Mol Biol & Microbiol, Orlando, FL 32816 USA |
| 推荐引用方式 GB/T 7714 | Fu, Ya-Yuan,Gao, Wen-Long,Chen, Mengqian,et al. Prostasin regulates human placental trophoblast cell proliferation via the epidermal growth factor receptor signaling pathway[J]. Human reproduction,2010,25(3):623-632. |
| APA | Fu, Ya-Yuan,Gao, Wen-Long,Chen, Mengqian,Chai, Karl X.,Wang, Yan-Ling,&Chen, Li-Mei.(2010).Prostasin regulates human placental trophoblast cell proliferation via the epidermal growth factor receptor signaling pathway.Human reproduction,25(3),623-632. |
| MLA | Fu, Ya-Yuan,et al."Prostasin regulates human placental trophoblast cell proliferation via the epidermal growth factor receptor signaling pathway".Human reproduction 25.3(2010):623-632. |
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来源：中国科学院大学
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