High glucose stimulates adipogenic and inhibits osteogenic differentiation in mg-63 cells through camp/protein kinase a/extracellular signal-regulated kinase pathway
文献类型：期刊论文
| 作者 | Wang, Weiwei; Zhang, Xiaolin; Zheng, Jiaqiang; Yang, Jianhong |
| 刊名 | Molecular and cellular biochemistry
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 338期号:1-2页码:115-122 |
| 关键词 | High glucose Osteogenic differentiation Adipogenic differentiation Camp Protein kinase a Extracellular signal-regulated kinase |
| ISSN号 | 0300-8177 |
| DOI | 10.1007/s11010-009-0344-6 |
| 通讯作者 | Yang, jianhong(yangjhong@yahoo.com.cn) |
| 英文摘要 | Patients with diabetes tend to have an increased incidence of osteoporosis that may be related to hyperglycemia. in this study, we investigated the effects of high glucose on differentiation of human osteoblastic mg-63 cells and involved intracellular signal transduction pathways. here, we showed that high glucose suppressed the cell growth, mineralization, and expression of osteogenic markers including runx2, collagen i, osteocalcin, osteonectin, but inversely promoted expression of adipogenic markers including ppar gamma, ap2, resistin, and adipsin. moreover, high glucose significantly increased the intracellular camp level in a time-dependent manner and induced erk1/2 activation. meanwhile, supplementation of h89, a specific inhibitor of pka, and pd98059, a specific inhibitor of mapk/erk kinase, reversed the cell growth inhibition, the down-regulation of osteogenic markers and the up-regulation of adipogenic markers as well as the activation of erk under high glucose. these results indicate that high glucose can increase adipogenic and inhibit osteogenic differentiation by activating camp/pka/erk pathway in mg-63 cells, thereby providing further insight into the molecular mechanism of diabetic osteoporosis. |
| WOS关键词 | MESENCHYMAL STEM-CELLS ; DIABETES-MELLITUS ; BONE LOSS ; EXPRESSION ; OSTEOBLASTS ; TRANSCRIPTION ; INDUCTION |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000276745400012 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2410673 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yang, Jianhong |
| 作者单位 | Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Weiwei,Zhang, Xiaolin,Zheng, Jiaqiang,et al. High glucose stimulates adipogenic and inhibits osteogenic differentiation in mg-63 cells through camp/protein kinase a/extracellular signal-regulated kinase pathway[J]. Molecular and cellular biochemistry,2010,338(1-2):115-122. |
| APA | Wang, Weiwei,Zhang, Xiaolin,Zheng, Jiaqiang,&Yang, Jianhong.(2010).High glucose stimulates adipogenic and inhibits osteogenic differentiation in mg-63 cells through camp/protein kinase a/extracellular signal-regulated kinase pathway.Molecular and cellular biochemistry,338(1-2),115-122. |
| MLA | Wang, Weiwei,et al."High glucose stimulates adipogenic and inhibits osteogenic differentiation in mg-63 cells through camp/protein kinase a/extracellular signal-regulated kinase pathway".Molecular and cellular biochemistry 338.1-2(2010):115-122. |
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来源：中国科学院大学
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