Hairpin dna probe based surface plasmon resonance biosensor used for the activity assay of e. coli dna ligase
文献类型：期刊论文
| 作者 | Luan, Qingfen1; Xue, Ying1; Yao, Xin1,2; Lu, Wu3 |
| 刊名 | Analyst
 |
| 出版日期 | 2010 |
| 卷号 | 135期号:2页码:414-418 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/b920228e |
| 通讯作者 | Yao, xin(yaox@gucas.ac.cn) |
| 英文摘要 | Using hairpin dna probe self-structure change during dna ligation process, a sensitive, label-free and simple method of e. coli dna ligase assay via a home-built high-resolution surface plasmon resonance (spr) instrument was developed. the dna ligation process was monitored in real-time and the effects of single-base mutation on the dna ligation process were investigated. then an assay of e. coli dna ligase was completed with a lower detection limit (0.6 nm), wider concentration range and better reproducibility. moreover, the influence of quinacrine on the activity of e. coli dna ligase was also studied, which demonstrated that our method was useful for drug screening. |
| WOS关键词 | SEQUENCE-SPECIFIC DETECTION ; ENERGY-TRANSFER ASSAY ; MOLECULAR BEACONS ; LIGATION ; OLIGONUCLEOTIDE ; HYBRIDIZATION ; SPECTROSCOPY ; MECHANISM ; REPAIR ; SENSOR |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000273921800025 |
| 出版者 | ROYAL SOC CHEMISTRY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2410990 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yao, Xin |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Coll Chem & Chem Engn, Beijing 100049, Peoples R China 2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China 3.Aerosp Res Inst Mat & Proc Technol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luan, Qingfen,Xue, Ying,Yao, Xin,et al. Hairpin dna probe based surface plasmon resonance biosensor used for the activity assay of e. coli dna ligase[J]. Analyst,2010,135(2):414-418. |
| APA | Luan, Qingfen,Xue, Ying,Yao, Xin,&Lu, Wu.(2010).Hairpin dna probe based surface plasmon resonance biosensor used for the activity assay of e. coli dna ligase.Analyst,135(2),414-418. |
| MLA | Luan, Qingfen,et al."Hairpin dna probe based surface plasmon resonance biosensor used for the activity assay of e. coli dna ligase".Analyst 135.2(2010):414-418. |
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来源：中国科学院大学
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