Development of expressed sequence tag-derived microsatellite markers for saccharina (laminaria) japonica
文献类型：期刊论文
| 作者 | Liu, Fuli1,2; Wang, Xiuliang1; Yao, Jianting1; Fu, Wandong1,2; Duan, Delin1 |
| 刊名 | Journal of applied phycology
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 22期号:1页码:109-111 |
| 关键词 | Phaeophyta Microsatellite Est Molecular marker |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-009-9426-9 |
| 通讯作者 | Duan, delin(dlduan@ms.qdio.ac.cn) |
| 英文摘要 | Expressed sequence tag-derived microsatellite markers (est-ssr) were generated and characterized in laminaria japonica using data mining from updated public est databases and polymorphism testing. fifty-eight of 578 ests (10.0%) containing various repeat motifs were used to design polymerase chain reaction (pcr) amplification primers. a total of 12 pairs of primer were generated and used in the pcr amplification. alleles per locus ranged from two to ten (average of 5.7). the observed heterozygosities and expected heterozygosities were from 0.045 to 0.543 and from 0.056 to 0.814, respectively. all loci were in hardy-weinberg equilibrium and no linkage disequilibrium was detected. these robust, informative, and potentially transferable polymorphic markers appear suitable for population, genetic, parentage, and mapping studies of l. japonica. |
| WOS关键词 | PHAEOPHYTA GAMETOPHYTES ; RAPD MARKERS ; DNA MARKERS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS类目 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000274435300013 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2411227 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Duan, Delin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100059, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Fuli,Wang, Xiuliang,Yao, Jianting,et al. Development of expressed sequence tag-derived microsatellite markers for saccharina (laminaria) japonica[J]. Journal of applied phycology,2010,22(1):109-111. |
| APA | Liu, Fuli,Wang, Xiuliang,Yao, Jianting,Fu, Wandong,&Duan, Delin.(2010).Development of expressed sequence tag-derived microsatellite markers for saccharina (laminaria) japonica.Journal of applied phycology,22(1),109-111. |
| MLA | Liu, Fuli,et al."Development of expressed sequence tag-derived microsatellite markers for saccharina (laminaria) japonica".Journal of applied phycology 22.1(2010):109-111. |
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来源：中国科学院大学
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