Identification and functional analysis of the genes encoding delta(6)-desaturase from ribes nigrum dagger
文献类型：期刊论文
| 作者 | Song, Li-Ying1,5; Lu, Wan-Xiang1,2; Hu, Jun1; Zhang, Yan1; Yin, Wei-Bo1; Chen, Yu-Hong1; Hao, Shan-Ting1; Wang, Bai-Lin3; Wang, Richard R-C4; Hu, Zan-Min1 |
| 刊名 | Journal of experimental botany
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 61期号:6页码:1827-1838 |
| ISSN号 | 0022-0957 |
| 关键词 | Delta(6)-desaturase Delta(8)-sphingolipid desaturase Functional analysis Ribes nigrum Subcellular localization |
| DOI | 10.1093/jxb/erq051 |
| 通讯作者 | Hu, zan-min(zmhu@genetics.ac.cn) |
| 英文摘要 | Gamma-linolenic acid (gamma-linolenic acid, gla; c18:3 delta(6, 9, 12)) belongs to the omega-6 family and exists primarily in several plant oils, such as evening primrose oil, blackcurrant oil, and borage oil. delta(6)-desaturase is a key enzyme involved in the synthesis of gla. there have been no previous reports on the genes encoding delta(6)-desaturase in blackcurrant (ribes nigrum l.). in this research, five nearly identical copies of delta(6)-desaturase gene-like sequences, named rnd8a, rnd8b, rnd6c, rnd6d, and rnd6e, were isolated from blackcurrant. heterologous expression in saccharomyces cerevisiae and/or arabidopsis thaliana confirmed that rnd6c/d/e were delta(6)-desaturases that could use both alpha-linolenic acids (ala; c18:3 delta(9,12,15)) and linoleic acid (la; c18:2 delta(9,12)) precursors in vivo, whereas rnd8a/b were delta(8)-sphlingolipid desaturases. expression of gfp tagged with rnd6c/d/e showed that blackcurrant delta(6)-desaturases were located in the mitochondrion (mit) in yeast and the endoplasmic reticulum (er) in tobacco. gc-ms results showed that blackcurrant accumulated gla and octadecatetraenoic acids (ota; c18:4 delta(6,9,12,15)) mainly in seeds and a little in other organs and tissues. rt-pcr results showed that rnd6c and rnd6e were expressed in all the tissues at a low level, whereas rnd6d was expressed at a high level only in seeds, leading to the accumulation of gla and ota in seeds. this research provides new insights to our understanding of gla synthesis and accumulation in plants and the evolutionary relationship of this class of desaturases, and new clues as to the amino acid determinants which define precise enzyme activity. |
| WOS关键词 | GAMMA-LINOLENIC ACID ; N-3 SUBSTRATE PREFERENCES ; CYTOCHROME B(5) DOMAIN ; FATTY-ACIDS ; BORAGO-OFFICINALIS ; HETEROLOGOUS EXPRESSION ; DELTA(6)-FATTY ACID ; DESATURASE ; PLANTS ; ENZYME |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000276735300023 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2412652 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Hu, Zan-Min |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400716, Peoples R China 3.Heilongjiang Agr Acad, Hort Div, Harbin 150069, Peoples R China 4.Utah State Univ, USDA ARS, FRRL, Logan, UT 84322 USA 5.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Li-Ying,Lu, Wan-Xiang,Hu, Jun,et al. Identification and functional analysis of the genes encoding delta(6)-desaturase from ribes nigrum dagger[J]. Journal of experimental botany,2010,61(6):1827-1838. |
| APA | Song, Li-Ying.,Lu, Wan-Xiang.,Hu, Jun.,Zhang, Yan.,Yin, Wei-Bo.,...&Hu, Zan-Min.(2010).Identification and functional analysis of the genes encoding delta(6)-desaturase from ribes nigrum dagger.Journal of experimental botany,61(6),1827-1838. |
| MLA | Song, Li-Ying,et al."Identification and functional analysis of the genes encoding delta(6)-desaturase from ribes nigrum dagger".Journal of experimental botany 61.6(2010):1827-1838. |
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来源：中国科学院大学
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