Molecular analysis of phosphomannomutase (pmm) genes reveals a unique pmm duplication event in diverse triticeae species and the main pmm isozymes in bread wheat tissues
文献类型：期刊论文
| 作者 | Yu, Chunmei1,2,3; Li, Yiwen1; Li, Bin1,3; Liu, Xin1; Hao, Lifang1,3; Chen, Jing1,3; Qian, Weiqiang1,3; Li, Shiming1,3; Wang, Guanfeng1,3; Bai, Shiwei1,3 |
| 刊名 | Bmc plant biology
 |
| 出版日期 | 2010-10-05 |
| 卷号 | 10页码:16 |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/1471-2229-10-214 |
| 通讯作者 | Wang, daowen(dwwang@genetics.ac.cn) |
| 英文摘要 | Background: phosphomannomutase (pmm) is an essential enzyme in eukaryotes. however, little is known about pmm gene and function in crop plants. here, we report molecular evolutionary and biochemical analysis of pmm genes in bread wheat and related triticeae species. results: two sets of homoeologous pmm genes (tapmm-1 and 2) were found in bread wheat, and two corresponding pmm genes were identified in the diploid progenitors of bread wheat and many other diploid triticeae species. the duplication event yielding pmm-1 and 2 occurred before the radiation of diploid triticeae genomes. the pmm gene family in wheat and relatives may evolve largely under purifying selection. among the six tapmm genes, the transcript levels of pmm-1 members were comparatively high and their recombinant proteins were all enzymatically active. however, pmm-2 homoeologs exhibited lower transcript levels, two of which were also inactive. tapmm-a1, b1 and d1 were probably the main active isozymes in bread wheat tissues. the three isozymes differed from their counterparts in barley and brachypodium distachyon in being more tolerant to elevated test temperatures. conclusion: our work identified the genes encoding pmm isozymes in bread wheat and relatives, uncovered a unique pmm duplication event in diverse triticeae species, and revealed the main active pmm isozymes in bread wheat tissues. the knowledge obtained here improves the understanding of pmm evolution in eukaryotic organisms, and may facilitate further investigations of pmm function in the temperature adaptability of bread wheat. |
| WOS关键词 | ACETYL-COA CARBOXYLASE ; HEAT-SHOCK-PROTEIN ; 3-PHOSPHOGLYCERATE KINASE ; PHYLOGENETIC ANALYSIS ; ARABIDOPSIS-THALIANA ; POSITIVE SELECTION ; POLYPLOID WHEAT ; HIGHER-PLANTS ; EVOLUTION ; RICE |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000283870500001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2412916 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Daowen |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Nantong Univ, Life Sci Sch, Nantong 226019, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Chunmei,Li, Yiwen,Li, Bin,et al. Molecular analysis of phosphomannomutase (pmm) genes reveals a unique pmm duplication event in diverse triticeae species and the main pmm isozymes in bread wheat tissues[J]. Bmc plant biology,2010,10:16. |
| APA | Yu, Chunmei.,Li, Yiwen.,Li, Bin.,Liu, Xin.,Hao, Lifang.,...&Wang, Daowen.(2010).Molecular analysis of phosphomannomutase (pmm) genes reveals a unique pmm duplication event in diverse triticeae species and the main pmm isozymes in bread wheat tissues.Bmc plant biology,10,16. |
| MLA | Yu, Chunmei,et al."Molecular analysis of phosphomannomutase (pmm) genes reveals a unique pmm duplication event in diverse triticeae species and the main pmm isozymes in bread wheat tissues".Bmc plant biology 10(2010):16. |
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来源：中国科学院大学
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