Production of mice using ips cells and tetraploid complementation
文献类型：期刊论文
| 作者 | Zhao, Xiao-Yang1,4; Lv, Zhuo1,4; Li, Wei1,4; Zeng, Fanyi2,3; Zhou, Qi1 |
| 刊名 | Nature protocols
 |
| 出版日期 | 2010 |
| 卷号 | 5期号:5页码:963-971 |
| ISSN号 | 1754-2189 |
| DOI | 10.1038/nprot.2010.61 |
| 通讯作者 | Zhou, qi(qzhou@ioz.ac.cn) |
| 英文摘要 | Induced pluripotent stem cells (ipscs) are considered to be an attractive alternative to embryonic stem cells (escs) and may provide great potential for clinical applications in regenerative medicine. although possessing characteristics similar to escs, the true pluripotency of these newly studied ipscs was not known because none of the previously developed ipscs passed the tetraploid complementation assay, which is regarded as the most stringent test for pluripotency. we have recently shown that by modifying some of the culture conditions for inducing ipscs, we were able to generate cell lines of high pluripotency, resulting in the production of live-born, fertile animals through tetraploid complementation. in this paper, we describe details of our methods of generating ips cell lines and subsequently producing full-term live animals through the tetraploid complementation assay; the procedure can be completed within 2 months. |
| WOS关键词 | PLURIPOTENT STEM-CELLS ; GENERATION ; EMBRYOS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemical Research Methods |
| 语种 | 英语 |
| WOS记录号 | WOS:000277174100015 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2413083 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhou, Qi |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 2.Shanghai Jiao Tong Univ, Inst Med Genet, Shanghai 200030, Peoples R China 3.Shanghai Jiao Tong Univ, Inst Med Sci, Shanghai 200030, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xiao-Yang,Lv, Zhuo,Li, Wei,et al. Production of mice using ips cells and tetraploid complementation[J]. Nature protocols,2010,5(5):963-971. |
| APA | Zhao, Xiao-Yang,Lv, Zhuo,Li, Wei,Zeng, Fanyi,&Zhou, Qi.(2010).Production of mice using ips cells and tetraploid complementation.Nature protocols,5(5),963-971. |
| MLA | Zhao, Xiao-Yang,et al."Production of mice using ips cells and tetraploid complementation".Nature protocols 5.5(2010):963-971. |
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来源：中国科学院大学
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