Bubr1 is a spindle assembly checkpoint protein regulating meiotic cell cycle progression of mouse oocyte
文献类型：期刊论文
| 作者 | Wei, Liang1,2; Liang, Xing-Wei1; Zhang, Qing-Hua1; Li, Mo1,2; Yuan, Ju1,2; Li, Sen1,2; Sun, Shao-Chen1; Ouyang, Ying-Chun1; Schatten, Heide3; Sun, Qing-Yuan1 |
| 刊名 | Cell cycle
 |
| 出版日期 | 2010-03-15 |
| 卷号 | 9期号:6页码:1112-1121 |
| 关键词 | Bubr1 Mouse oocyte Spindle assembly checkpoint Meiosis maturation Kinetochore-microtubule attachments |
| ISSN号 | 1538-4101 |
| 通讯作者 | Sun, qing-yuan(sunqy@ioz.ac.cn) |
| 英文摘要 | Bubr1 (bub1-related kinase or mad3/bub1b) is an essential component of the spindle assembly checkpoint ( sac) and plays an important role in kinetochore localization of other spindle checkpoint proteins in mitosis. but its roles in mammalian oocyte meiosis are unclear. in the present study, we examined the expression, localization and function of bubr1 during mouse oocyte meiotic maturation. the expression level of bubr1 increased progressively from germinal vesicle to metaphase ii stages. immunofluorescent analysis showed that bubr1 localized to kinetochores from the germinal vesicle breakdown to the prometaphase i stages, co-localizing with polo-like kinase 1, while it disappeared from the kinetochores at the metaphase i stage. spindle disruption by nocodazole treatment caused relocation of bubr1 to kinetochores at metaphase i, anaphase i and metaphase ii stages; spindle microtubules were disrupted by low temperature treatment in the bubr1-depleted oocytes in meiosis i, suggesting that bubr1 monitors kinetochore-microtubule (k-mt) attachments. overexpression of exogenous bubr1 arrested oocyte meiosis maturation at the m i stage or earlier; in contrast, dominant-negative bubr1 and bubr1 depletion accelerated meiotic progression. in the bubr1-depleted oocytes, higher percentage of chromosome misalignment was observed and more oocytes overrode the m i stage arrest induced by low concentration of nocodazole. our data suggest that bubr1 is a spindle assembly checkpoint protein regulating meiotic progression of oocytes. |
| WOS关键词 | KINETOCHORE-MICROTUBULE INTERACTIONS ; MITOTIC CHECKPOINT ; CHROMOSOME SEGREGATION ; CHROMATID SEPARATION ; MAMMALIAN OOCYTES ; I ARREST ; MEIOSIS ; LOCALIZATION ; INHIBITION ; COMPLEX |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000275636900018 |
| 出版者 | LANDES BIOSCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2413371 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, Qing-Yuan |
| 作者单位 | 1.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA |
| 推荐引用方式 GB/T 7714 | Wei, Liang,Liang, Xing-Wei,Zhang, Qing-Hua,et al. Bubr1 is a spindle assembly checkpoint protein regulating meiotic cell cycle progression of mouse oocyte[J]. Cell cycle,2010,9(6):1112-1121. |
| APA | Wei, Liang.,Liang, Xing-Wei.,Zhang, Qing-Hua.,Li, Mo.,Yuan, Ju.,...&Sun, Qing-Yuan.(2010).Bubr1 is a spindle assembly checkpoint protein regulating meiotic cell cycle progression of mouse oocyte.Cell cycle,9(6),1112-1121. |
| MLA | Wei, Liang,et al."Bubr1 is a spindle assembly checkpoint protein regulating meiotic cell cycle progression of mouse oocyte".Cell cycle 9.6(2010):1112-1121. |
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来源：中国科学院大学
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