Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana
文献类型：期刊论文
| 作者 | Ying, Xiao-Bao1,2,3; Dong, Li1,2; Zhu, Hui1,2,3; Duan, Cheng-Guo1,2; Du, Quan-Sheng1,2; Lv, Dian-Qiu1,2,4; Fang, Yuan-Yuan1,2; Antonio Garcia, Juan5; Fang, Rong-Xiang1,2; Guo, Hui-Shan1,2 |
| 刊名 | Plant cell
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 22期号:4页码:1358-1372 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.109.072058 |
| 通讯作者 | Guo, hui-shan(guohs@im.ac.cn) |
| 英文摘要 | Endogenous eukaryotic rna-dependent rna polymerases (rdrs) produce double-stranded rna intermediates in diverse processes of small rna synthesis in rna silencing pathways. rdr6 is required in plants for posttranscriptional gene silencing induced by sense transgenes (s-ptgs) and has an important role in amplification of antiviral silencing. whereas rdr1 is also involved in antiviral defense in plants, this does not necessarily proceed through triggering silencing. in this study, we show that nicotiana benthamiana transformed with rdr1 from nicotiana tabacum (nt-rdr1 plants) exhibits hypersusceptibility to plum pox potyvirus and other viruses, resembling rdr6-silenced (rdr6i) n. benthamiana. analysis of transient induction of rna silencing in n. benthamiana nt-rdr1 and rdr6i plants revealed that nt-rdr1 possesses silencing suppression activity. we found that nt-rdr1 does not interfere with rdr6-dependent sirna accumulation but turns out to suppress rdr6-dependent s-ptgs. our results, together with previously published data, suggest that rdr1 might have a dual role, contributing, on one hand, to salicylic acid-mediated antiviral defense, and suppressing, on the other hand, the rdr6-mediated antiviral rna silencing. we propose a scenario in which the natural loss-of-function variant of rdr1 in n. benthamiana may be the outcome of selective pressure to maintain a high rdr6-dependent antiviral defense, which would be required to face the hypersensitivity of this plant to a large number of viruses. |
| WOS关键词 | SMALL INTERFERING RNAS ; TOBACCO-MOSAIC-VIRUS ; DIRECTED DNA METHYLATION ; TRANS-ACTING SIRNAS ; SALICYLIC-ACID ; C-ELEGANS ; CAENORHABDITIS-ELEGANS ; ARABIDOPSIS-THALIANA ; ANTIVIRAL DEFENSE ; HELICASE DOMAIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000278414900029 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2413538 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Guo, Hui-Shan |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Heilongjiang Acad Agr Sci, Virus Free Seedling Inst, Haerbin 150086, Heilongjiang, Peoples R China 5.CSIC, Ctr Natl Biotecnol, Dept Plant Mol Genet, E-28049 Madrid, Spain |
| 推荐引用方式 GB/T 7714 | Ying, Xiao-Bao,Dong, Li,Zhu, Hui,et al. Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana[J]. Plant cell,2010,22(4):1358-1372. |
| APA | Ying, Xiao-Bao.,Dong, Li.,Zhu, Hui.,Duan, Cheng-Guo.,Du, Quan-Sheng.,...&Guo, Hui-Shan.(2010).Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana.Plant cell,22(4),1358-1372. |
| MLA | Ying, Xiao-Bao,et al."Rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana".Plant cell 22.4(2010):1358-1372. |
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来源：中国科学院大学
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