Novel immunodominant peptide presentation strategy: a featured hla-a*2402-restricted cytotoxic t-lymphocyte epitope stabilized by intrachain hydrogen bonds from severe acute respiratory syndrome coronavirus nucleocapsid protein
文献类型：期刊论文
| 作者 | Liu, Jun1,2,3; Wu, Peng4; Gao, Feng5; Qi, Jianxun1,2; Kawana-Tachikawa, Ai6; Xie, Jing4; Vavricka, Christopher J.1; Iwamoto, Aikichi6,7; Li, Taisheng4; Gao, George F.1,2,3,8 |
| 刊名 | Journal of virology
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 84期号:22页码:11849-11857 |
| ISSN号 | 0022-538X |
| DOI | 10.1128/jvi.01464-10 |
| 通讯作者 | Gao, george f.(gaof@im.ac.cn) |
| 英文摘要 | Antigenic peptides recognized by virus-specific cytotoxic t lymphocytes (ctls) are presented by major histocompatibility complex (mhc; or human leukocyte antigen [hla] in humans) molecules, and the peptide selection and presentation strategy of the host has been studied to guide our understanding of cellular immunity and vaccine development. here, a severe acute respiratory syndrome coronavirus (sars-cov) nucleocapsid (n) protein-derived ctl epitope, n1 (qfkdnvill), restricted by hla-a*2402 was identified by a series of in vitro studies, including a computer-assisted algorithm for prediction, stabilization of the peptide by co-refolding with hla-a*2402 heavy chain and beta 2-microglobulin (beta(2)m), and t2-a24 cell binding. consequently, the antigenicity of the peptide was confirmed by enzyme-linked immunospot (elispot), proliferation assays, and hla-peptide complex tetramer staining using peripheral blood mononuclear cells (pbmcs) from donors who had recovered from sars donors. furthermore, the crystal structure of hla-a*2402 complexed with peptide n1 was determined, and the featured peptide was characterized with two unexpected intrachain hydrogen bonds which augment the central residues to bulge out of the binding groove. this may contribute to the t-cell receptor (tcr) interaction, showing a host immunodominant peptide presentation strategy. meanwhile, a rapid and efficient strategy is presented for the determination of naturally presented ctl epitopes in the context of given hla alleles of interest from long immunogenic overlapping peptides. |
| WOS关键词 | CELL-RECEPTOR RECOGNITION ; SARS CORONAVIRUS ; CTL EPITOPES ; VACCINE CANDIDATES ; RECOVERED PATIENTS ; CRYSTAL-STRUCTURES ; STRUCTURAL BASIS ; SPIKE PROTEIN ; CLASS-I ; IDENTIFICATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000283420500024 |
| 出版者 | AMER SOC MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2413817 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, George F. |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Mol Microbiol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Coll Life Sci, Beijing 100049, Peoples R China 4.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Infect Dis, Beijing 100730, Peoples R China 5.Chinese Acad Sci, Inst Biophys IBP, Beijing 100101, Peoples R China 6.Univ Tokyo, Res Hosp, Adv Clin Res Ctr,Div Infect Dis, Dept Infect Dis & Appl Immunol,Minato Ku, Tokyo 1088639, Japan 7.Univ Tokyo, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan 8.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jun,Wu, Peng,Gao, Feng,et al. Novel immunodominant peptide presentation strategy: a featured hla-a*2402-restricted cytotoxic t-lymphocyte epitope stabilized by intrachain hydrogen bonds from severe acute respiratory syndrome coronavirus nucleocapsid protein[J]. Journal of virology,2010,84(22):11849-11857. |
| APA | Liu, Jun.,Wu, Peng.,Gao, Feng.,Qi, Jianxun.,Kawana-Tachikawa, Ai.,...&Gao, George F..(2010).Novel immunodominant peptide presentation strategy: a featured hla-a*2402-restricted cytotoxic t-lymphocyte epitope stabilized by intrachain hydrogen bonds from severe acute respiratory syndrome coronavirus nucleocapsid protein.Journal of virology,84(22),11849-11857. |
| MLA | Liu, Jun,et al."Novel immunodominant peptide presentation strategy: a featured hla-a*2402-restricted cytotoxic t-lymphocyte epitope stabilized by intrachain hydrogen bonds from severe acute respiratory syndrome coronavirus nucleocapsid protein".Journal of virology 84.22(2010):11849-11857. |
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来源：中国科学院大学
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