Low Spring Constant Regulates P-Selectin-Psgl-1 Bond Rupture
文献类型：期刊论文
| 作者 | Zhang Y(章燕) ; Sun GY(孙淦云); Lv SQ(吕守芹); Li N(李宁); Long M(龙勉)
|
| 刊名 | Biophysical Journal
 |
| 出版日期 | 2008 |
| 页码 | 5439-5448 |
| 通讯作者邮箱 | mlong@imech.ac.cn |
| 关键词 | Selectin Glycoprotein Ligand-1 P-Selectin Cell-Adhesion Binding-Kinetics Force Dissociation Lifetime Flow Microscope Leukocytes |
| ISSN号 | 0006-3495 |
| 通讯作者 | Long, MA (reprint author), Chinese Acad Sci, Natl Micrograv Lab, Beijing 100190, Peoples R China. |
| 中文摘要 | Forced dissociation of selectin-ligand bonds is crucial to such biological processes as leukocyte recruitment, thrombosis formation, and tumor metastasis. Although the bond rupture has been well known at high loading rate r(f) (>= 10(2) pN/s), defined as the product of spring constant k and retract velocity v, how the low r(f) (< 10(2) pN/s) or the low k regulates the bond dissociation remains unclear. Here an optical trap assay was used to quantify the bond rupture at r(f) <= 20 pN/s with low k (similar to 10(-3)-10(-2) pN/nm) when P-selectin and P-selectin glycoprotein ligand 1 (PSGL-1) were respectively coupled onto two glass microbeads. Our data indicated that the bond rupture force f retained the similar values when r(f) increased up to 20 pN/s. It was also found that f varied with different combinations of k and v even at the same r(f). The most probable force, f |
| 类目[WOS] | Biophysics |
| 研究领域[WOS] | Biophysics |
| 关键词[WOS] | SELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CELL-ADHESION ; BINDING-KINETICS ; FORCE ; DISSOCIATION ; LIFETIME ; FLOW ; MICROSCOPE ; LEUKOCYTES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000260999500042 |
| 公开日期 | 2009-08-03 ; 2009-09-14 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/25826]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Zhang Y,Sun GY,Lv SQ,et al. Low Spring Constant Regulates P-Selectin-Psgl-1 Bond Rupture[J]. Biophysical Journal,2008:5439-5448. |
| APA | 章燕,孙淦云,吕守芹,李宁,&龙勉.(2008).Low Spring Constant Regulates P-Selectin-Psgl-1 Bond Rupture.Biophysical Journal,5439-5448. |
| MLA | 章燕,et al."Low Spring Constant Regulates P-Selectin-Psgl-1 Bond Rupture".Biophysical Journal (2008):5439-5448. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。