Combody: one-domain antibody multimer with improved avidity
文献类型：期刊论文
| 作者 | Zhu, Xuekai2; Wang, Lei2; Liu, Rongzhi; Flutter, Barry3; Li, Shenghua4; Ding, Jie2; Tao, Hua; Liu, Changzhen; Sun, Meiyi5; Gao, Bin1,6 |
| 刊名 | Immunology and cell biology
 |
| 出版日期 | 2010-08-01 |
| 卷号 | 88期号:6页码:667-675 |
| 关键词 | Antibody engineering Avidity Multivalency Single-domain antibody Tcr-like antibody |
| ISSN号 | 0818-9641 |
| DOI | 10.1038/icb.2010.21 |
| 通讯作者 | Gao, bin(bgao2004@gmail.com) |
| 英文摘要 | Antibodies (abs) have been engineered into small antigen-binding fragments and rebuilt into multivalent high-avidity molecules for improving in vivo pharmacokinetics and efficacy in clinical use. to increase the avidity of a t-cell receptor-like single-domain ab (sdab) specific for hla-a2 complex, we fused the sdab to a coiled-coil peptide derived from human cartilage oligomeric matrix protein (comp48) to make an sdab multimer, termed combody. the combody improved the binding avidity of sdab significantly, whereas the specificity for the targeted cells was retained. the strategy was also expanded to create a bispecific combody by fusing an sdab to the n-terminal and an anti-cd3 single-chain variable fragment to the c-terminal of comp48. the dual-specific combody was able to efficiently mediate cytotoxicity against the target cells in vitro. taken together, the strategy to make combody could be widely adopted to increase the avidity of ab fragment for further application. immunology and cell biology (2010) 88, 667-675; doi: 10.1038/icb.2010.21; published online 9 march 2010 |
| WOS关键词 | ALPHA-HELICAL PROTEIN ; MONOCLONAL-ANTIBODY ; COILED-COIL ; IN-VIVO ; CHIMERIC ANTIBODY ; ESCHERICHIA-COLI ; LIGHT-CHAINS ; V-H ; FRAGMENTS ; ANTIGEN |
| WOS研究方向 | Cell Biology ; Immunology |
| WOS类目 | Cell Biology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000280830700012 |
| 出版者 | NATURE PUBLISHING GROUP |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2414403 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, Bin |
| 作者单位 | 1.Chinese Acad Sci, Ctr Mol Immunol, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100101, Peoples R China 3.UCL Inst Child Hlth, London, England 4.Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada 5.Epigen Biotec, Beijing, Peoples R China 6.Chinese Acad Sci, China Japan Joint Lab Mol Immunol & Microbiol, Inst Microbiol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Xuekai,Wang, Lei,Liu, Rongzhi,et al. Combody: one-domain antibody multimer with improved avidity[J]. Immunology and cell biology,2010,88(6):667-675. |
| APA | Zhu, Xuekai.,Wang, Lei.,Liu, Rongzhi.,Flutter, Barry.,Li, Shenghua.,...&Gao, Bin.(2010).Combody: one-domain antibody multimer with improved avidity.Immunology and cell biology,88(6),667-675. |
| MLA | Zhu, Xuekai,et al."Combody: one-domain antibody multimer with improved avidity".Immunology and cell biology 88.6(2010):667-675. |
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来源：中国科学院大学
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