Intergenic and repeat transcription in human, chimpanzee and macaque brains measured by rna-seq
文献类型：期刊论文
| 作者 | Xu, Augix Guohua1,2; He, Liu1,3; Li, Zhongshan1,3; Xu, Ying1; Li, Mingfeng1; Fu, Xing1; Yan, Zheng1; Yuan, Yuan1; Menzel, Corinna4; Li, Na5 |
| 刊名 | Plos computational biology
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 6期号:7页码:10 |
| ISSN号 | 1553-734X |
| DOI | 10.1371/journal.pcbi.1000843 |
| 通讯作者 | Xu, augix guohua() |
| 英文摘要 | Transcription is the first step connecting genetic information with an organism's phenotype. while expression of annotated genes in the human brain has been characterized extensively, our knowledge about the scope and the conservation of transcripts located outside of the known genes' boundaries is limited. here, we use high-throughput transcriptome sequencing (rna-seq) to characterize the total non-ribosomal transcriptome of human, chimpanzee, and rhesus macaque brain. in all species, only 20-28% of non-ribosomal transcripts correspond to annotated exons and 20-23% to introns. by contrast, transcripts originating within intronic and intergenic repetitive sequences constitute 40-48% of the total brain transcriptome. notably, some repeat families show elevated transcription. in non-repetitive intergenic regions, we identify and characterize 1,093 distinct regions highly expressed in the human brain. these regions are conserved at the rna expression level across primates studied and at the dna sequence level across mammals. a large proportion of these transcripts (20%) represents 3'utr extensions of known genes and may play roles in alternative microrna-directed regulation. finally, we show that while transcriptome divergence between species increases with evolutionary time, intergenic transcripts show more expression differences among species and exons show less. our results show that many yet uncharacterized evolutionary conserved transcripts exist in the human brain. some of these transcripts may play roles in transcriptional regulation and contribute to evolution of human-specific phenotypic traits. |
| WOS关键词 | GENE-EXPRESSION PATTERNS ; 3' UNTRANSLATED REGIONS ; NONCODING RNAS ; MESSENGER-RNAS ; EVOLUTION ; GENOMES ; REVEALS ; CELLS ; DATABASE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Mathematical & Computational Biology |
| WOS类目 | Biochemical Research Methods ; Mathematical & Computational Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000280528300012 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2414558 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Xu, Augix Guohua |
| 作者单位 | 1.Partner Inst Computat Biol, Shanghai, Peoples R China 2.Max Planck Inst Evolutionary Anthropol, Leipzig, Germany 3.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China 4.Max Planck Inst Mol Genet, Berlin, Germany 5.Berlin Inst Med Syst Biol, Max Delbruck Centrum Mol Med, Berlin, Germany |
| 推荐引用方式 GB/T 7714 | Xu, Augix Guohua,He, Liu,Li, Zhongshan,et al. Intergenic and repeat transcription in human, chimpanzee and macaque brains measured by rna-seq[J]. Plos computational biology,2010,6(7):10. |
| APA | Xu, Augix Guohua.,He, Liu.,Li, Zhongshan.,Xu, Ying.,Li, Mingfeng.,...&Khaitovich, Philipp.(2010).Intergenic and repeat transcription in human, chimpanzee and macaque brains measured by rna-seq.Plos computational biology,6(7),10. |
| MLA | Xu, Augix Guohua,et al."Intergenic and repeat transcription in human, chimpanzee and macaque brains measured by rna-seq".Plos computational biology 6.7(2010):10. |
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来源：中国科学院大学
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