Genes encoding the alpha-carboxyltransferase subunit of acetyl-coa carboxylase from brassica napus and parental species: cloning, expression patterns, and evolution
文献类型：期刊论文
| 作者 | Li, Zhi-Guo1,2; Yin, Wei-Bo1; Guo, Huan1,2; Song, Li-Ying1,2; Chen, Yu-Hong1; Guan, Rong-Zhan3; Wang, Jing-Qiao4; Wang, Richard R. -C.5; Hu, Zan-Min1 |
| 刊名 | Genome
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 53期号:5页码:360-370 |
| 关键词 | Brassica Acetyl-coa carboxylase (accase) Alpha-ct Evolution |
| ISSN号 | 0831-2796 |
| DOI | 10.1139/g10-011 |
| 通讯作者 | Hu, zan-min(zmhu@genetics.ac.cn) |
| 英文摘要 | Heteromeric acetyl coenzyme a carboxylase (accase), a rate-limiting enzyme in fatty acid biosynthesis in dicots, is a multi-enzyme complex consisting of biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase (alpha-ct and beta-ct). in the present study, four genes encoding alpha-ct were cloned from brassica napus, and two were cloned from each of the two parental species, b. rapa and b. oleracea. comparative and cluster analyses indicated that these genes were divided into two major groups. the major divergence between group-1 and group-2 occurred in the second intron. group-2 alpha-ct genes represented the ancestral form in the genus brassica. the divergence of group-1 and group-2 genes occurred in their common ancestor 12.96-17.78 million years ago (mya), soon after the divergence of arabidopsis thaliatza and brassica (15-20 mya). this time of divergence is identical to that reported for the paralogous subgenomes of diploid brassica species (13-17 mya). real-time reverse transcription pcr revealed that the expression patterns of the two groups of genes were similar in different organs, except in leaves. to better understand the regulation and evolution of a-ct genes, promoter regions from two sets of orthologous gene copies from b. napus, b. rapa, and b. oleracea were cloned and compared. the function of the promoter of gene bars-ct-i-i in group-1 and gene bna-ct-2-i in group-2 was examined by assaying p-glucuronidase activity in transgenic a. thaliana. our results will be helpful in elucidating the evolution and regulation of accase in oilseed rape. |
| WOS关键词 | COENZYME-A CARBOXYLASE ; ARABIDOPSIS-THALIANA ; BIOTIN CARBOXYLASE ; DIACYLGLYCEROL ACYLTRANSFERASE ; NUCLEOTIDE SUBSTITUTION ; LIPID BIOSYNTHESIS ; MOLECULAR-CLONING ; CARRIER PROTEIN ; GENOME ; PLANTS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000279243900004 |
| 出版者 | NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2414658 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Hu, Zan-Min |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China 4.Yunnan Agr Acad, Inst Econ Crops, Kunming 65023, Peoples R China 5.Utah State Univ, USDA, ARS, Forage & Range Res Lab, Logan, UT 84322 USA |
| 推荐引用方式 GB/T 7714 | Li, Zhi-Guo,Yin, Wei-Bo,Guo, Huan,et al. Genes encoding the alpha-carboxyltransferase subunit of acetyl-coa carboxylase from brassica napus and parental species: cloning, expression patterns, and evolution[J]. Genome,2010,53(5):360-370. |
| APA | Li, Zhi-Guo.,Yin, Wei-Bo.,Guo, Huan.,Song, Li-Ying.,Chen, Yu-Hong.,...&Hu, Zan-Min.(2010).Genes encoding the alpha-carboxyltransferase subunit of acetyl-coa carboxylase from brassica napus and parental species: cloning, expression patterns, and evolution.Genome,53(5),360-370. |
| MLA | Li, Zhi-Guo,et al."Genes encoding the alpha-carboxyltransferase subunit of acetyl-coa carboxylase from brassica napus and parental species: cloning, expression patterns, and evolution".Genome 53.5(2010):360-370. |
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来源：中国科学院大学
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