Enzyme biosensor based on nad-sensitive quantum dots
文献类型：期刊论文
| 作者 | Ren, Xiangling1,2; Yang, Liuqing1,2,3; Tang, Fangqiong1,2; Yan, Chuanmiao1,2,3; Ren, Jun1,2 |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2010-09-15 |
| 卷号 | 26期号:1页码:271-274 |
| 关键词 | Quantum dots Fluorescence Biosensors Lactate dehydrogenase Nicotinamide adenine dinucleotide |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2010.05.014 |
| 通讯作者 | Tang, fangqiong() |
| 英文摘要 | A novel quantum dots (qds) based biosensor has been developed to detect the activity of lactate dehydrogenase (ldh) by the change of fluorescence intensities of the qds in this system, the fluorescence intensities of the qds are quenched by nicotinamide adenine cinucleotide (nad. the coenzyme of ldh) first and then intensified with increasing amounts of the ldh because of the consumption of the nad in the biocatalyzed reaction a linear calibration plot of the activity of ldh is obtained in the wide amounts range from 150 to 1500 u/l and the detection limit is 75 u/l furthermore, the effect of several possible interfering substances, such as uric acid (ua), lactose, and glucose on the qds-based biosensor is also investigated. this enzyme biosensor is of considerable interest due to its promise for simple procedure and the established method has great potential in detection of the physiological level of some biomolecules in clinical diagnostics of various diseases. (c) 2010 elsevier b v. all rights reserved |
| WOS关键词 | RESONANCE ENERGY-TRANSFER ; LACTATE-DEHYDROGENASE ; CANCER CELLS ; HYBRIDIZATION ; GLUCOSE ; CDTE ; NANOCRYSTALS ; DONORS ; ACID ; PH |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000282560500043 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2414890 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tang, Fangqiong |
| 作者单位 | 1.Chinese Acad Sci, Tech Inst Phys & Chem, Lab Controllable Preparat & Applicat Nanomat, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Xiangling,Yang, Liuqing,Tang, Fangqiong,et al. Enzyme biosensor based on nad-sensitive quantum dots[J]. Biosensors & bioelectronics,2010,26(1):271-274. |
| APA | Ren, Xiangling,Yang, Liuqing,Tang, Fangqiong,Yan, Chuanmiao,&Ren, Jun.(2010).Enzyme biosensor based on nad-sensitive quantum dots.Biosensors & bioelectronics,26(1),271-274. |
| MLA | Ren, Xiangling,et al."Enzyme biosensor based on nad-sensitive quantum dots".Biosensors & bioelectronics 26.1(2010):271-274. |
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来源：中国科学院大学
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