Integrated profiling of micrornas and mrnas: micrornas located on xq27.3 associate with clear cell renal cell carcinoma
文献类型：期刊论文
| 作者 | Zhou, Liang1; Chen, Jiahao2,3; Li, Zhizhong2,3; Li, Xianxin1; Hu, Xueda2,4,5; Huang, Yi1; Zhao, Xiaokun6; Liang, Chaozhao7; Wang, Yong1; Sun, Liang1 |
| 刊名 | Plos one
 |
| 出版日期 | 2010-12-30 |
| 卷号 | 5期号:12页码:10 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0015224 |
| 通讯作者 | Zhou, liang() |
| 英文摘要 | Background: with the advent of second-generation sequencing, the expression of gene transcripts can be digitally measured with high accuracy. the purpose of this study was to systematically profile the expression of both mrna and mirna genes in clear cell renal cell carcinoma (ccrcc) using massively parallel sequencing technology. methodology: the expression of mrnas and mirnas were analyzed in tumor tissues and matched normal adjacent tissues obtained from 10 ccrcc patients without distant metastases. in a prevalence screen, some of the most interesting results were validated in a large cohort of ccrcc patients. principal findings: a total of 404 mirnas and 9,799 mrnas were detected to be differentially expressed in the 10 ccrcc patients. we also identified 56 novel mirna candidates in at least two samples. in addition to confirming that canonical cancer genes and mirnas (including vegfa, dusp9 and erbb4; mir-210, mir-184 and mir-206) play pivotal roles in ccrcc development, promising novel candidates (such as pnck and mir-122) without previous annotation in ccrcc carcinogenesis were also discovered in this study. pathways controlling cell fates (e. g., cell cycle and apoptosis pathways) and cell communication (e. g., focal adhesion and ecm-receptor interaction) were found to be significantly more likely to be disrupted in ccrcc. additionally, the results of the prevalence screen revealed that the expression of a mirna gene cluster located on xq27.3 was consistently downregulated in at least 76.7% of similar to 50 ccrcc patients. conclusions: our study provided a two-dimensional map of the mrna and mirna expression profiles of ccrcc using deep sequencing technology. our results indicate that the phenotypic status of ccrcc is characterized by a loss of normal renal function, downregulation of metabolic genes, and upregulation of many signal transduction genes in key pathways. furthermore, it can be concluded that downregulation of mirna genes clustered on xq27.3 is associated with ccrcc. |
| WOS关键词 | EXPRESSION PROFILES ; GENE-EXPRESSION ; E-CADHERIN ; REPRESSORS ZEB1 ; BREAST-CANCER ; HYPOXIA ; PROGNOSIS ; PHENOTYPE ; SIGNATURE ; TARGETS |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000285793600014 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2415318 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhou, Liang |
| 作者单位 | 1.Peking Univ, Key Lab Stem Cell Biol, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Inst Urol,Shenzhen Hosp,Shenzhen PKU HKUST Med Ct, Shenzhen, Peoples R China 2.Beijing Genom Inst Shenzhen, Shenzhen, Peoples R China 3.S China Univ Technol, Sch Biosci & Biotechnol, Guangzhou, Guangdong, Peoples R China 4.Chinese Acad Sci, Beijing Inst Genom, Beijing, Peoples R China 5.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 6.Cent So Univ, Dept Urosurg, Hosp 2, Changsha, Hunan, Peoples R China 7.Anhui Med Univ, Dept Urosurg, Hosp 1, Hefei, Peoples R China 8.Shantou Univ, Coll Med, Shantou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Liang,Chen, Jiahao,Li, Zhizhong,et al. Integrated profiling of micrornas and mrnas: micrornas located on xq27.3 associate with clear cell renal cell carcinoma[J]. Plos one,2010,5(12):10. |
| APA | Zhou, Liang.,Chen, Jiahao.,Li, Zhizhong.,Li, Xianxin.,Hu, Xueda.,...&Zhang, Xiuqing.(2010).Integrated profiling of micrornas and mrnas: micrornas located on xq27.3 associate with clear cell renal cell carcinoma.Plos one,5(12),10. |
| MLA | Zhou, Liang,et al."Integrated profiling of micrornas and mrnas: micrornas located on xq27.3 associate with clear cell renal cell carcinoma".Plos one 5.12(2010):10. |
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来源：中国科学院大学
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