Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured rpe cells
文献类型：期刊论文
| 作者 | Wu, Hui-Juan; Li, Xiao-Xin; Dong, Jian-Qiang; Pei, Wei-Hua; Chen, Hong-Da |
| 刊名 | Graefes archive for clinical and experimental ophthalmology
 |
| 出版日期 | 2007 |
| 卷号 | 245期号:1页码:135-142 |
| 关键词 | Retinal pigment epithelium (rpe) Subretinal microphotodiode array (mpda) Biocompatibility Primary culture Microporous filter |
| ISSN号 | 0721-832X |
| DOI | 10.1007/s00417-006-0296-4 |
| 通讯作者 | Li, xiao-xin(drlixiaoxin@vip.sina.com) |
| 英文摘要 | Background: subretinal microphotodiode array (mpda) is a type of visual prosthesis used for the implantation in the subretinal space of patients with progressive photoreceptor cell loss. the present study aimed to evaluate the effect of materials for mpda on the viability, apoptosis and barrier function of cultured pig retinal pigment epithelium (rpe) cells. methods: primary culture of pig rpe cells was performed and 24 pig eyes were used to start rpe culture. the third passage of the cultures was plated on different materials for mpda and mpdas. the tetrazolium dye-reduction assay (mtt) was used to determine rpe cell viability. flow cytometry was measured to indicate the apoptosis rates of rpe cells on different materials. rpe cells were also cultured on microporous filters, and the transepithelial resistance and permeability of the experimental molecule were measured to determine the barrier function. results: the data from all the methods indicated no significant difference between the materials groups and the control group, and the materials tested showed good biocompatibility. conclusions: the materials for mpda used in the present study had no direct toxicity to the rpe cells and did not release harmful soluble factors that affected the barrier function of rpe in vitro. |
| WOS关键词 | PIGMENT EPITHELIAL-CELLS ; ANNEXIN-V ; EXPRESSION ; ARPE-19 ; LINE |
| WOS研究方向 | Ophthalmology |
| WOS类目 | Ophthalmology |
| 语种 | 英语 |
| WOS记录号 | WOS:000242461600017 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2427127 |
| 专题 | 半导体研究所 |
| 通讯作者 | Li, Xiao-Xin |
| 作者单位 | 1.Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing, Peoples R China 2.Peking Univ, Dept Pathol, Peoples Hosp, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Hui-Juan,Li, Xiao-Xin,Dong, Jian-Qiang,et al. Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured rpe cells[J]. Graefes archive for clinical and experimental ophthalmology,2007,245(1):135-142. |
| APA | Wu, Hui-Juan,Li, Xiao-Xin,Dong, Jian-Qiang,Pei, Wei-Hua,&Chen, Hong-Da.(2007).Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured rpe cells.Graefes archive for clinical and experimental ophthalmology,245(1),135-142. |
| MLA | Wu, Hui-Juan,et al."Effects of subretinal implant materials on the viability, apoptosis and barrier function of cultured rpe cells".Graefes archive for clinical and experimental ophthalmology 245.1(2007):135-142. |
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来源：半导体研究所
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