Partial transfection of cells using laminar flows in microchannels
文献类型：期刊论文
| 作者 | Li, Lei1,2; Nie, Yong3; Shi, Xuetao1; Wu, Hongkai1; Ye, Datian4,5; Chen, Hongda2 |
| 刊名 | Biomicrofluidics
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 5期号:3页码:8 |
| 关键词 | Biomems Cellular transport Fluorescence Gene therapy Genetics Glass Laminar flow Lipid bilayers Microchannel flow Molecular biophysics Polymers Proteins |
| ISSN号 | 1932-1058 |
| DOI | 10.1063/1.3643827 |
| 通讯作者 | Wu, hongkai(chhkwu@ust.hk) |
| 英文摘要 | This manuscript describes a convenient method for partial transfection using a y-shaped microchannel polydimethylsiloxane (pdms)-glass chip and on-chip cationic lipid-mediated transfection. enhanced green fluorescent protein genes (pegfp-n2) were introduced into the cos-7 cells cultured in half of the channel, while red fluorescent protein genes (pdsred-n1) were introduced into the cells cultured in another half of the channel. this on-chip partial transfection technique provides an avenue for the spatial control of transfection. it is possible to use this technique to perform parallel transfection on chips in order to study cell behaviors under two or more gene transfections in the same culture. (c) 2011 american institute of physics. [doi: 10.1063/1.3643827] |
| WOS关键词 | MAMMALIAN-CELLS ; CHIP ; ELECTROPORATION ; CHEMOTAXIS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Science & Technology - Other Topics ; Physics |
| WOS类目 | Biochemical Research Methods ; Biophysics ; Nanoscience & Nanotechnology ; Physics, Fluids & Plasmas |
| 语种 | 英语 |
| WOS记录号 | WOS:000295618500036 |
| 出版者 | AMER INST PHYSICS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2428198 |
| 专题 | 半导体研究所 |
| 通讯作者 | Wu, Hongkai |
| 作者单位 | 1.Tohoku Univ, WPI Adv Inst Mat Res, Aoba Ku, Sendai, Miyagi 9808577, Japan 2.Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing 100083, Peoples R China 3.Peking Univ, Coll Engn, Dept Energy & Resources Engn, Beijing 100871, Peoples R China 4.Tsinghua Univ, Sch Med, Dept Biomed Engn, Beijing 100084, Peoples R China 5.Tsinghua Univ, Grad Sch Shenzhen, Biomed Engn Res Ctr, Shenzhen 518055, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Lei,Nie, Yong,Shi, Xuetao,et al. Partial transfection of cells using laminar flows in microchannels[J]. Biomicrofluidics,2011,5(3):8. |
| APA | Li, Lei,Nie, Yong,Shi, Xuetao,Wu, Hongkai,Ye, Datian,&Chen, Hongda.(2011).Partial transfection of cells using laminar flows in microchannels.Biomicrofluidics,5(3),8. |
| MLA | Li, Lei,et al."Partial transfection of cells using laminar flows in microchannels".Biomicrofluidics 5.3(2011):8. |
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来源：半导体研究所
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