H+isfet-based biosensor for determination of penicillin g
文献类型：期刊论文
| 作者 | Liu, JG; Liang, L; Li, GX; Han, RS; Chen, KM |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 1998-10-15 |
| 卷号 | 13期号:9页码:1023-1028 |
| 关键词 | H- ion sensitive field effect transistor (h+-isfet) Penicillin g acylase Enzyme based fet biosensor (enfet) Determination of penicillin g |
| ISSN号 | 0956-5663 |
| 通讯作者 | Liu, jg() |
| 英文摘要 | A biosensor based on an h+ ion sensitive field effect transistor (h+-isfet) and penicillin g acylase has been developed. the response time of the sensor to different concentrations of penicillin g was 30 s. in a 20 mm phosphate buffer at ph 7.0, the linear range of the calibration curve was from 0.5 to 8 mm. the coefficients of variation for three samples with 20 repeated measurements were below 5%. stability of the sensor could reach about 6 months and more than 1000 runs were performed without a significant decrease of the output value. the sensor was tested for measurement of the penicillin g content in penicillin fermentation broth. forty samples with low and high concentrations of penicillin g were chosen for the correlation test. the values assayed by the sensor method were compared with the values assayed by hplc method, the correlation coefficient (r) was 0.9944 and the regression equation was y = 1.034x - 2083.7 respectively. the different measuring methods are discussed in the text. (c) 1998 published by elsevier science s.a. all rights reserved. |
| WOS关键词 | FIELD-EFFECT TRANSISTOR ; ENZYME POTENTIOMETRIC SENSORS ; ELECTRODES ; ISFET |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000076848800011 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2428612 |
| 专题 | 半导体研究所 |
| 通讯作者 | Liu, JG |
| 作者单位 | 1.Acad Sinica, Inst Microbiol, Beijing 100080, Peoples R China 2.Acad Sinica, Inst Semicond, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, JG,Liang, L,Li, GX,et al. H+isfet-based biosensor for determination of penicillin g[J]. Biosensors & bioelectronics,1998,13(9):1023-1028. |
| APA | Liu, JG,Liang, L,Li, GX,Han, RS,&Chen, KM.(1998).H+isfet-based biosensor for determination of penicillin g.Biosensors & bioelectronics,13(9),1023-1028. |
| MLA | Liu, JG,et al."H+isfet-based biosensor for determination of penicillin g".Biosensors & bioelectronics 13.9(1998):1023-1028. |
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来源：半导体研究所
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