A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space
文献类型：期刊论文
| 作者 | Sun SJ(孙树津) ; Gao YX(高宇欣); Shu NJ; Tang ZM(唐泽眉); Tao ZL(陶祖莱); Long M(龙勉)
|
| 刊名 | Microgravity Science And Technology
 |
| 出版日期 | 2008 |
| 页码 | 115-120 |
| 通讯作者邮箱 | sunshj@imech.ac.cn ; mlong@imech.ac.cn |
| 关键词 | Counter Sheet-Flow Sandwich Cell Culture Mammalian Cell Growth Microgravity Dogfish Scyliorhinus Stellaris Shear-Stress Spaceflight Microgravity Bioreactors Exchange Gills Blood Water Wall |
| ISSN号 | 0938-0108 |
| 通讯作者 | Sun, SJ (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China. |
| 中文摘要 | Cell culture and growth in space is crucial to understand the cellular responses under microgravity. The effects of microgravity were coupled with such environment restrictions as medium perfusion, in which the underlying mechanism has been poorly understood. In the present work, a customer-made counter sheet-flow sandwich cell culture device was developed upon a biomechanical concept from fish gill breathing. The sandwich culture unit consists of two side chambers where the medium flow is counter-directional, a central chamber where the cells are cultured, and two porous polycarbonate membranes between side and central chambers. Flow dynamics analysis revealed the symmetrical velocity profile and uniform low shear rate distribution of flowing medium inside the central culture chamber, which promotes sufficient mass transport and nutrient supply for mammalian cell growth. An on-orbit experiment performed on a recovery satellite was used to validate the availability of the device. |
| 类目[WOS] | Engineering, Aerospace ; Thermodynamics ; Mechanics |
| 研究领域[WOS] | Engineering ; Thermodynamics ; Mechanics |
| 关键词[WOS] | DOGFISH SCYLIORHINUS STELLARIS ; SHEAR-STRESS ; SPACEFLIGHT ; MICROGRAVITY ; BIOREACTORS ; EXCHANGE ; GILLS ; BLOOD ; WATER ; WALL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000260252100009 |
| 公开日期 | 2009-08-03 ; 2009-09-14 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/25860]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Sun SJ,Gao YX,Shu NJ,et al. A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space[J]. Microgravity Science And Technology,2008:115-120. |
| APA | 孙树津,高宇欣,Shu NJ,唐泽眉,陶祖莱,&龙勉.(2008).A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space.Microgravity Science And Technology,115-120. |
| MLA | 孙树津,et al."A Novel Counter Sheet-Flow Sandwich Cell Culture Device For Mammalian Cell Growth In Space".Microgravity Science And Technology (2008):115-120. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。