Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS
文献类型：期刊论文
| 作者 | You, Jinmao ; Zhao, Huaixin ; Sun, Zhiwei ; Xia, Lian ; Yan, Tao ; Suo, Yourui ; Li, Yulin |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 ; You, JM; Zhao, HX; Sun, ZW; Xia, L; Yan, T; Suo, YR; Li, YL.Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS,JOURNAL OF SEPARATION SCIENCE,2009,32(9):1351-1362
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| 出版日期 | 2009-05-01 |
| 英文摘要 | A simple, sensitive method for the determination of aliphatic amines based on a sulfonylation reaction using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as pre-column labeling reagent with fluorescence detection and APCI-MS identification has been developed. The labeled derivatives exhibited high stability and were enough to be efficiently analyzed by HPLC with an excitation maximum at lambda(ex) 270 nm and an emission maximum at lambda(em) 430 nm. Identification of derivatives was carried out by online post-column MS in positive-ion mode. Comparing with the widely used 5-dimethylaminonaphthalene-1-sulfonylchloride (Dansyl-Cl), EASC-amine derivatives not only exhibited high fluorescence but also exhibited excellent MS ionizable potential. Detection limits obtained from 0.10 pmol injection, at a S/N of 3, were 4.0-12.7 fmol. The mean intra- and inter-assay precision for all aliphatic amine levels were <3.84 and 3.21%, respectively. Excellent linear responses were observed with coefficients of >0.9995.; A simple, sensitive method for the determination of aliphatic amines based on a sulfonylation reaction using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as pre-column labeling reagent with fluorescence detection and APCI-MS identification has been developed. The labeled derivatives exhibited high stability and were enough to be efficiently analyzed by HPLC with an excitation maximum at lambda(ex) 270 nm and an emission maximum at lambda(em) 430 nm. Identification of derivatives was carried out by online post-column MS in positive-ion mode. Comparing with the widely used 5-dimethylaminonaphthalene-1-sulfonylchloride (Dansyl-Cl), EASC-amine derivatives not only exhibited high fluorescence but also exhibited excellent MS ionizable potential. Detection limits obtained from 0.10 pmol injection, at a S/N of 3, were 4.0-12.7 fmol. The mean intra- and inter-assay precision for all aliphatic amine levels were <3.84 and 3.21%, respectively. Excellent linear responses were observed with coefficients of >0.9995. |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1878]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | You, Jinmao,Zhao, Huaixin,Sun, Zhiwei,et al. Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS[J]. JOURNAL OF SEPARATION SCIENCE, You, JM; Zhao, HX; Sun, ZW; Xia, L; Yan, T; Suo, YR; Li, YL.Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS,JOURNAL OF SEPARATION SCIENCE,2009,32(9):1351-1362,2009. |
| APA | You, Jinmao.,Zhao, Huaixin.,Sun, Zhiwei.,Xia, Lian.,Yan, Tao.,...&Li, Yulin.(2009).Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS.JOURNAL OF SEPARATION SCIENCE. |
| MLA | You, Jinmao,et al."Application of 10-ethyl-acridine-3-sulfonyl chloride for HPLC determination of aliphatic amines in environmental water using fluorescence and APCI-MS".JOURNAL OF SEPARATION SCIENCE (2009). |
入库方式： OAI收割
来源：西北高原生物研究所
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