A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering
文献类型：期刊论文
| 作者 | Zhang, Cunfang ; Xu, Kun ; Hu, Linyong ; Wang, Ling ; Zhang, Tingting ; Ren, Chonghua ; Zhang, Zhiying ; Zhang, ZY (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China. |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2015-02-01 |
| 英文摘要 | Genome editing with engineered nucleases, such as zinc-finger nucleases (ZFNs) and TALE nucleases, remains confronted with a high risk of cellular toxicity induced by off-targeting. Here we describe the construction of a suicidal nuclease expression vector in which a pair of ZFNs genes were flanked of its target sites. To further enrich the targeted cells, the suicidal ZFN expression cassette was also inserted within an eGFP reporter, to disrupt the ORF of eGFP gene. ZFN-associated toxicity was reduced by similar to 40 % with this new system, and the activities of ZFNs were similar to 4.5 % lower. We conclude that using this new suicidal ZFN expression and surrogate reporter system represents an improvement for genomic editing by reducing toxicity and allowing easy detection of edited cells by eGFP analysis.; Genome editing with engineered nucleases, such as zinc-finger nucleases (ZFNs) and TALE nucleases, remains confronted with a high risk of cellular toxicity induced by off-targeting. Here we describe the construction of a suicidal nuclease expression vector in which a pair of ZFNs genes were flanked of its target sites. To further enrich the targeted cells, the suicidal ZFN expression cassette was also inserted within an eGFP reporter, to disrupt the ORF of eGFP gene. ZFN-associated toxicity was reduced by similar to 40 % with this new system, and the activities of ZFNs were similar to 4.5 % lower. We conclude that using this new suicidal ZFN expression and surrogate reporter system represents an improvement for genomic editing by reducing toxicity and allowing easy detection of edited cells by eGFP analysis. |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5457]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, Cunfang,Xu, Kun,Hu, Linyong,et al. A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering[J]. BIOTECHNOLOGY LETTERS,2015. |
| APA | Zhang, Cunfang.,Xu, Kun.,Hu, Linyong.,Wang, Ling.,Zhang, Tingting.,...&Zhang, ZY .(2015).A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering.BIOTECHNOLOGY LETTERS. |
| MLA | Zhang, Cunfang,et al."A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering".BIOTECHNOLOGY LETTERS (2015). |
入库方式： OAI收割
来源：西北高原生物研究所
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